Notes: Heregulin is a growth factor that binds to the HER/ErbB family of receptors, which includes four HER members. HER1 and HER2 overexpression in breast cancer cells is associated with higher malignancy and poorer prognosis. However, HER4 expression is associated with longer survival and more positive prognosis. The authors of this study investigated HER4-dependent growth inhibition that is mediated by heregulin. The authors determined the absolute levels of HER4 mRNA in several human breast cancer and mouse cancer cell lines. For this determination, relative cell numbers were determined using a CellTiter® AQ_ueous MTS assay. Apoptosis in heregulin-treated or untreated cells was also determined using the DeadEnd™ Colorimetric TUNEL System. The authors concluded that heregulin decreases growth of HER4-positive breast cancer cells, and that this growth inhibition is dependent on BRCA1. (3596)

Notes: Basic FGF was glycosylated by non-enzymatic means and tested for HUVEC cell proliferation. The proliferation was measured with the procedure of the CellTiter 96^® AQ_ueous Non-Radioactive Assay using reagents prepared for MTS Powder (Promega) and phenazine methosulphate (PMS; Sigma). (0621)

Notes: The CellTiter 96^® AQ_ueous One Solution Cell Proliferation Assay was used to assess the survival of COS-7 cells transfected with the leutinizing hormone-releasing hormone receptor. Seventy-two hours after exposure to cytotoxic compounds the cell viability was measured in relation to control cultures. (1253)

Notes: The CellTiter 96^® AQ_ueous Assay (MTS Reagent) was used to measure the cytotoxic activity of wildtype and mutant soluble Fas ligand (sFasL) on murine B lymphoma A20 cells and human T lymphoblastoma Jurkat cells. (1706)

Notes: The CellTiter 96^® AQueous MTS Reagent Powder was used for assays to measure interferon alpha2a (IFN-alpha2a) inhibition of cytopathic effects (CPE) caused by a challenge of encephalomyocarditis (EMC) virus on WISH cells. (2097)

Notes: CellTiter 96^® AQueous MTS Powder and the CellTiter 96^® AQueous Non-Radioactive Cell Proliferation Assay were used to optimize an assay to test the sensitivity of TR146 human buccal epithelial cells treated with a series of beta-adrenoceptor antagonists. Parameters optimized for their screening assay included: initial cell seeding density, PMS concentration, MTS concentration, and stability of the MTS-formazan. (2090)