Liu, D., Li, C., Chen, Y., Burnett, C., Liu, X.Y., Downs, S., Collins, R.D., and Hawiger, J.
Notes: The Caspase-Glo® 3/7, -8 and -9 Assays were used to measure Caspase-3, -8, and -9 activities in mouse liver homogenates. The authors describe a modified procedure for making liver homogenates in a hypotonic extraction buffer. Homogenates were cleared with a 15 minute spin at 13,000 x g and normalized to a 1mg/ml concentration before use the assays. Data comparing control and cSN50 peptide-treated animals at various time points are presented. (3213)