Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Dickeya zeae. (4327)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Campylobacter coli. (4316)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Bartonella. (4314)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Campylobacter jejuni. (4317)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Anabaena sp. (4299)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Fusobacterium nucleatum. (4335)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Borrelia burgdorferi. (4315)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Acinetobacter baumannii. (4274)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Desulfitobacterium hafniense. (4326)

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Babesia canis. (4311)

Notes: These authors used the Maxwell® 16 Tissue DNA Purification Kit to extract DNA from 200µl samples of raw meat homogenate from beef, pork, poultry and lamb samples. They also used the Wizard® Genomic DNA Isolation System to extract DNA from cooked meat samples. The extracted DNA was used in real-time, quantitative PCR assays to identify species-specific DNA spiked at 1% in mixed DNA samples. (4353)

Notes: Wizard® Genomic DNA Purification Kit was used to extract DNA from Aspergillus sojae for GS FLX titanium fragment sequencing. (4542)

Notes: These authors compared the performance of four DNA purification methods for recovery of bacterial and archaeal DNA from fecal material. One of the methods tested was the Wizard® Genomic DNA Purification Kit, which uses a solution-based, enzymatic method for extraction. The Wizard® Genomic method was rated highly on extraction speed, and gave the highest DNA yields. A second method involving mechanical disruption (repeated bead beating) was rated more highly on extraction efficiency from archaea and some bacterial species. The criteria for performance comparison are described fully in the paper. (4219)

Notes: These authors investigated the differences in gut microbial flora between obese and normal-weight subjects. They used the Wizard® Genomic DNA Purification Kit to extract DNA from diluted fecal extracts. The extracted DNA was analyzed by PCR to identify Bacteroidetes and Firmicutes. Differences in distribution of these phyla was between the subject groups were identified. (4220)

Notes: Certain bacteriophages can promote host cell sporulation under unfavorable conditions to increase survival of the host and prophage. These types of phages, known as spore-converting phages, have been found in terrestrial Bacillus species. In this article the authors examined the effect of prophages on sporulation of 11 Bacillus isolates from the Gulf of Mexico. Potential prophages in the Bacillus isolates were detected by PCR using unique PCR primer sets for each prophage genome and GoTaq® Green Master Mix. One of these isolates, B14905, was examined in more detail; the genome of this isolate was isolated using the Wizard® Genomic DNA Purification Kit, then sequenced. (4091)

Notes: This paper explored the effect of methyl-CpGmodified single-stranded oligonucleotides (ssODN) on gene repair. The Wizard^® Genomic DNA Purification Kit was used to isolate gDNA from methylCpG-ssODN-treated myoblasts derived from limb muscle of neonatal C57 mice that stably expressed the mismatch repair site. One microgram of purified gDNA was digested overnight with 5U of restriction enzyme, purified, resuspended in 20µl of water and 5µl used in real-time PCR to determine if the target had been repaired. (4062)

Notes: The authors were interested on confirming the identity of the exhumed skeletal remains thought to be Nicolaus Copernicus. DNA isolation from teeth was performed by pulverizing the tooth in liquid nitrogen, soaking the powdered sample in 0.5M EDTA, 5% SDS and 3 mg proteinase K at 37 °C and extracting genomic DNA using the Wizard^® Genomic DNA Purification Kit. Bone and other tooth samples were extracted using another method and the Wizard^® Genomic DNA Purification Kit used for a salting-out method. Purified DNA was subjected to mitochondrial, autosomal and Y chromosome STR amplification and analysis. (4063)

Notes: The authors examined the role of thymidine kinase (TK) in establishing a herpesvirus infection via the upper respiratory tract. DNA was purified from ex vivo organs of female BALB/c mice infected with a murid herpesvirus-4 (MuHV-4) TK knockout using the Wizard^® Genomic DNA Purification Kit. Real-time PCR was used with 50–80ng of purified DNA to determine viral load of the animals. (4015)

Notes: In this paper, the researchers describe and demonstrate a new method for creating precise genome modifications in Saccharomyces cerevisiae. The mutagenic inverted repeat assisted genome engineering (MIRAGE) was tested in S. cerevisiae W303a by deleting gal7 as well as point and frameshift mutations. Genomic DNA was isolated using the Wizard^® Genomic DNA Purification Kit, amplified and modifications verified by gel analysis or DNA sequencing. (4014)

Notes: To examine the prevalence of Campylobacter species in asymptomatic carriers that can pass the bacteria onto humans, rectal swabs were collected from 147 dogs and 35 cats in Ireland and cultured on various diagnostic plates. The Wizard^® Genomic DNA Purification System was used to isolate DNA from any suspect Campylobacter cultures. The purified DNA was used in multiplex PCR and RFLP to determine which species of Campylobacter was present. (4016)

Notes: The association of thiopurines (anticancer drugs) with acute skin sensitivity to ultraviolet A (UVA) radiation and a high risk of skin cancer was tested using six human fibroblast and lymphoid cell lines. The thiopurine 6-thioguanine (6-TG) was added at 0.8 or 0.6mM to each of six cell lines and incubated for 48 hours to ensure incorporation. DNA and RNA were extracted and 40µg of nucleic acid were digested to nucleosides, separated by HPLC, and the 6-TG 20-deoxy and ribonucleosides quantified by absorbance at 342 nm. The same DNA isolation and digestion method was used when the cells were treated with 1µM of 6-TG and then irradiated with 5 kJ/m² UVA after 48 hours. The Wizard^® Genomic DNA Purification Kit was used for DNA extraction. (4013)

Notes: The authors developed a new human prostate cancer cell line, CWR22Pc, that is both androgen-dependent and able to produce tumors in dihydrotestosterone-supplemented nude mice. To confirm that CWR22Pc cells are derived from primary CWR22 human prostate xenograft tumors, the authors performed genotyping at 8 STR loci and amelogenin using the PowerPlex^® 1.2 System. DNA purification from the cell line and original tumor samples was performed using the Wizard^® Genomic DNA Purification Kit. (4041)

Notes: These authors investigated the amo operon of the marine ammonia oxidizer Nitrosococcus oceani. The bacteria were grown at 30°C for 3 weeks in 200-400ml batch cultures in artificial seawater in the dark without shaking. Genomic DNA was isolated from cells in stationary phase using the Wizard^® Genomic DNA Purification Kit. The isolated DNA was then used for PCR analysis. (3740)

Notes: Genomic DNA from Arabidopsis thaliana was isolated using the Wizard® Genomic DNA Purification Kit. The extracted DNA was used to amplify the 3´ UTR of AteIF5A-2, A. thaliana translation initiation factor 5A, or the entire gene for creating transgenic plants. Leaf discs from wild-type Arabidopsis were infected with Pseudomonas syringae pv tomato DC3000, a virulent strain regulated by AteIF5A-2, using a syringe. After 24 and 72 hours, 0.4cm leaf discs were fixed, labeled using the DeadEnd™ Fluorometric TUNEL System and stained for AteIF5A-2 protein. (3979)

Notes: The authors examined strains of Salmonella enterica serovar Typhi isolated from typhoid cases originating in or around Indonesia or from travelers returning from Indonesia to examine if serovar Typhi from this area has a greater level of genetic diversity compared to other countries. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit, diluted to 4 ng/µl and used in locus-specific PCR genotyping. (3980)