Notes: Promega's pGEM®-T Vector System, pCI Mammalian Expression Vector and TNT® Coupled Reticulocyte Lysate System were used in this study. (1951)

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM^®-T Vector. RNA probes were produced from the pGEM^®-T Vector for in situ hybridization studies. (1547)

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM^®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM^®-T Vector and sequenced. (1419)

Notes: The pGEM^®-T Vector System was used in this study. (2009)

Notes: Promega's pGEM®-T Vector System and TNT® Coupled Reticulocyte Lysate System were used in this study. (1948)

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM^®-T Vector System. (0529)

Notes: The authors use the Reverse Transcription System, Wizard^® PCR Preps DNA Purification Systems, Wizard^® Plus Minipreps DNA Purification System and the pGEM^®-T Vector System in their studies. (0445)

Notes: PCR products generated by differential display analysis were subcloned into the pGEM^®-T Vector System and subsequently sequenced. (0240)

Notes: The 3'-RACE products were cloned with the pGEM^®-T Vector System. (0673)

Notes: The pTargeT™ Vector was used to subclone the 2.5kb Na+/H+ Exchanger 2 and the 2.7kb Na+/H+ Exchanger 3 directly from PCR reactions. The constructs were stably transfected into PS120 Chinese hamster lung fibroblasts and selected for resistance to the neomycin analog, G-418. After 7-10 days of selection, the cells were further tested for expression by RT-PCR and immunoblotting. (1318)

Notes: The pGEM^®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

Notes: The pGEM^®-T Vector System was used in this study. (1969)

Notes: PCR products were cloned into pGEM^®-T Vector System. (0250)

Notes: PolyATtract^® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM^®-T Vector. (0748)

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM^®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

Notes: The AQP8 protein was expressed in vitro using the TNT^® Coupled Reticulocyte Lysate System in the presence of Canine Microsomal Membranes. The protein demonstrated an increase in molecular weight in the presence of the membranes. Promega's pGEM^®-T Vector System and Proteinase K were also used. (1624)

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM^®-T Vector System. (0998)

Notes: To identify the true transcriptional start of the IL-16 mRNA methods for rapid amplification of cDNA ends were applied. The complete pro-IL-16 cDNA was cloned, sequenced, and expressed in COS-7 cells. The authors used the pGEM^®-T Vector System to clone fragments that were then used as templates in the Riboprobe^® in Vitro Transcription System. (1453)

Notes: Point mutations in the repeated portion of the PKD1 gene were detected by the Protein Truncation Test. PCR products containing the T7 promoter were used as templates and protein was labeled by incorporation of [³H] leucine in a transcription/translation reaction using TNT^® Coupled Reticulocyte Lysate System. The pGEM^®-T Vector System was also used. (0489)

Notes: PolyA⁺ RNA was isolated from rat total RNA using the PolyATtract^® mRNA Isolation System and used for Northern analysis. The pGEM^®-T Vector System was used to clone products from RT-PCR. (0447)

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract^® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM^®-T Vector and sequenced. (1491)

Notes: The pCI Mammalian Expression Vector was used to express the p64H1 protein in 293 cells and HT-4, a clonal neuronal cell line. Inserts in the vectors were verified for expression using the TNT^® Coupled Reticulocyte Lysate System. The proteins were translated in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The pGEM^®-T Vector System was used to clone the PCR product of circle rapid amplification of cDNA ends to find the true 5'-end of the p64H1 cDNA. The Tfx™-20 Reagent was used to transfect 293 cells and HT-4 neuronal cells with the p64H1-expressing pCI-based plasmid. (1206)

Notes: The pGEM^®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)