Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay carbamide peroxide mediated cytotoxicity. (2980)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to investigate the protective effects of an antioxidant (pycnogenol) on oxidant (t-butyl hydroperoxide) induced injury of bovine pulmonary artery endothelial cells. Cellular injury was assessed both by determining release of LDH using the CytoTox 96^® Assay System and by measuring cell viability using MTT. (1751)

Notes: These authors describe a SURALISA as a rapid, easy, and highly sensitive assay to quantify Cu/Zn superoxide dismutase released from damaged cells into the supernatant. The specificity of the monoclonal antibodies used, which react exclusively with human SOD, prevent binding of bovine SOD present in FCS. The authors compared their system to the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay ONLY on the basis of single cell lysis and looked at sensitivity and background values (i.e., they did not perform cell-mediated cytotoxicity experiments in this technical note). (1741)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to study toxicity in hippocampal neurons. (2228)

Notes: A short rapid communication where the authors use the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay to measure cell death. HEK 293 cells were transfected with various plasmids containing the respective NMDA receptor subunit cDNAs by the calcium phosphate method. (1738)

Notes: Using a PC-12 clone, the authors assessed Beta toxicity by three assays; MTT reduction, LDH release (CytoTox^® 96 Non-Radioactive Cytotoxicity Assay) and visual counting with trypan blue. Research focuses on antioxidant activity on the amyloid beta protein which accumulates in the central nervous system in Alzheimer's disease. Please note that these authors did not use the CytoTox 96^® Assay for cell-mediated cytotoxicity, however it is an interesting article looking at mechanisms of toxicity. (1736)

Notes: The authors compared the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay to ⁵¹Cr and found the assays were equally sensitive and gave identical results. All cytotoxicity data shown are based on LDH release measurements. EBV-transformed B cell lines, flu peptide specific effector cells also were used (all human lines and cells). (1737)

Notes: The authors found LDH release to be a simple means to assess glutamate mediated central neuronal cell injury of cortical cells (containing both neuronal and glial elements from fetal mice). They measured LDH by following NADH at 340nm (i.e., non-colorimetric) and found that LDH is extremely stable, even without serum, in culture medium when stored at 37°C. The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to measure LDH release. (1746)