Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay activated mouse splenocyte mediated cytotoxicity. (2955)

Notes: The authors used the CytoTox 96® Non-Radioactive Cytotoxicity Assay system to study cytoprotection of human gastric cells. (0876)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay Shigella mediated cytotoxicity. (2939)

Notes: Rather than detect cytotoxicity, the CytoTox 96^® Non-Radioactive Cytotoxicity Assay System was used to quantitate cell number. Cells were first reacted with a fluorescein dye to detect reactive oxygen species (ROS), then the cells were lysed and total LDH determined. The data was reported as ROS/LDH. Studies were performed in SH-SY5Y neuroblastomas depleted of their mitochondria by removing its mitochondrial DNA. These cells were fused with platelets obtained from Alzheimer's patients and disease-free controls. (0387)

Notes: In this study, the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to study antibody dependent cell-mediated cytotoxicity (ADCC). ARH-77 cells, plated at 2 x 10⁴ cells per well in a 96-well plate, were used as target cells. Antibody or fusion proteins were added to the cells at 1μg/ml and ratios of 12.5:1 to 50:1 effector:target cells were made with MNC (bone marrow mononuclear) cells or PNC (neutrophilic polymorphonuclear) leukocytes. Plates were incubated at 37°C for 4 hours, after which supernatants were analyzed for LDH release. (3014)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay activated human PBMC mediated cytotoxicity. (2866)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to measure the cytolytic activity of bacterial toxins on cell lines and primary cultures of midgut epithelial cells from Spodoptera sp. insects. The LDH assay conditions were optimized for studying insect cell cytotoxicity. (1757)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay System was used to demonstrate that mild heat shock (42°C for 3hr) and severe heat shock (45° for 1hr) did not cause cytotoxicity of LLC-PK1 porcine renal epithelial cells. Cells under the heat shock conditions release approximately 1% of maximal LDH. (0323)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to measure the cytotoxicity of human neuronal-like SH-SY5Y cells. The authors demonstrated that inhibition of p75 TNFR using antisense oligonucleotides increases the cytotoxicity of neurotypic cells to hypoxic injury, TNFalpha, and beta-Amyloid treatments. (1753)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to examine the LDH released from PC12 cells treated with β-amyloid peptides. The LDH measurements were performed with cell supernatants that also had been used for an MTT cell proliferation assay. (1040)

Notes: The CytoTox® 96 Non-Radioactive Cytotoxicity Assay was used to measure LDH release from rat PC12 pheochromocytoma cells treated with beta-amyloid peptides (Abeta). The authors demonstrated Abeta peptides increase the sensitivity of cells to insults such as MTT formazan formation and that phloretin decreases the susceptibility of the plasma membrane to damage induced by Abeta peptide. (1742)

Notes: Various compounds were tested on ECV304 human endothelial cells and Jurkat cells. The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to ensure that the test compounds did not cause cytotoxicity. Authors report that N-acetyl L-cysteine interferes with the system and could not be used with this treatment. (2241)

Notes: THP-1 cells were the targets of monocytes activated with a number of different substances and monitored with the CytoTox 96^® Cytotoxicity Assay to monitor LDH release. (0721)

Notes: The Cytotox 96^® Non-Radioactive Cytotoxicity Assay was used to monitor the cytotoxicity of the human osteoblast-like cell line , MG63, to peptides derived from Mycobacterium tuberculosis chaperonin 10 protein. (0717)

Notes: The Cytotox 96^® Non-Radioactive Cytotoxicity Assay was used to assess the cytotoxicity (LDH release) of COS-7 SV40-transformed monkey kidney cells with regard to lipid-based transfection reagents. (0217)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used as a sensitive assay of cell adhesion. Adhesive cells were lysed in a Triton^® X-100 solution and the lysates assayed for LDH activity. (0878)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used in conjunction with MTT and DNA quantitation to monitor the growth of the rat pituitary tumor cell line GH3 treated with thyroid hormones. (1743)

Notes: Authors investigated in a variety of parameters the toxicity of benzol peroxide on a nontumorigenic human epidermal keratinocyte RHEK-1 cell line. Membrane integrity (LDH release measured using the CytoTox 96^® Non-Radioactive Cytotoxicity Assay) was studied following brief exposure to these compounds. (1734)

Notes: Investigators used the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay to look at the effect of sanguinarine chloride on membrane integrity in variety of cell lines and primary cells from oral human tissue. Human cell lines tested were Smulow-Glickman (S-G), HGF-1 gingival fibroblasts and the KB human carcinoma cell line. (2911)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to measure complement-mediated cytotoxicity with COS-7 and pig kidney PK15 cells. Instead of measuring the LDH released from the injured cells, they quantified the amount of LDH activity retained in the undamaged cells. (1752)

Notes: The CytoTox^® 96 Non-Radioactive Cytotoxicity Assay was used to monitor the lysis of J774A.1 macrophages infected with various strains of Bordetella pertussis. (1745)

Notes: The effect of the phosphorothioate oligodeoxynucleotide S-dC28 on the proliferation of vascular smooth muscle cells in the presence or absence of PDGF was assessed using the CellTiter 96® AQ_ueous Non-Radioactive Cell Proliferation Assay. Additionally, LDH release was monitored using the CytoTox 96® Non-Radioactive Cytotoxicity Assay to determine if S-dC28 was directly cytotoxic to vascular smooth muscle cells. (2549)

Notes: In describing their modifications for a fluorescent dye label cytotoxicity assay, the authors compared it to Promega's CytoTox® 96 Non-Radioactive Cytotoxicity Assay. With 4 hour NK activity experiments, the results (% cytotoxicity) obtained by flow cytometry agreed well with the results obtained by the CytoTox 96® Assay. They also investigated extended periods of incubation, because in some domestic animal models, NK activity requires an overnight incubation for lysis to occur. For long assays (16–20 hours of co-culture), there were instances when the LDH assay did not give meaningful data. In particular, PBMLs isolated from some individual animals yielded a high proportion of porcine PBML cells that died during this overnight assay. However, certain experiments were successful (better PBML overnight survival) and good correlation was observed with the CytoTox 96® Assay and their flow cytometry assay. (1744)

Notes: Authors used the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay to measure cytolytic activity of expanded CTLs on LDV-infected macrophages. They indicate previous problems in measuring lysis of LDV infected macrophages by conventional ⁵¹Cr release. Evidently there isn't enough time to label the cells following virus infection before the cells die. Also, because the macrophages are extremely difficult to culture, they wanted to maintain assay conditions in the presence of 10% FBS. To decrease the culture medium background, they surveyed various lots of fetal bovine serum, contacted the manufacturer and ultimately chose a particular lot with low endogenous LDH. (1739)

Notes: The authors investigated the toxicity of carbamide peroxide in a variety of cell types by various parameters, including LDH release. The article includes data showing toxicity with human Smulow-Glickman gingival epithelial cells quantitated by LDH release (CytoTox^® 96 Non-Radioactive Cytotoxicity Assay). (1755)