Notes: Apoptosis was studied by TUNEL assay using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) and PI staining. Amount of apoptosis was quantitated by flow cytometry. Test compounds that activate the TLR2 pathway were tested for cytotoxicity using the CytoTox 96^® Non-Radioactive Cytotoxicity Assay. The cells were at 2.4x10⁴ cells/well. (2128)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay glutamate receptor agonist mediated cytotoxicity. (2868)

Notes: 293 cells stably expressing the human toll-like receptor-2 were challenged with various agents and analyzed for apoptosis. One test was a visual inspection for blebbing and the other was quantitation by TUNEL staining, using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay), followed by FACS analysis. In another assay for THP-1 cell death, the CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to monitor LDH release in response to bacterial lipoproteins. (1508)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay 8-bromo-cAMP mediated cytotoxicity. (2975)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay hydrogen Peroxide; Angeli's salt, and Diethylamine-NONOate mediated cytotoxicity. (2943)

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess cell lysis after pulse chase experiments were used to assay secretion of chimeric proteins. (2888)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay activated human T cells mediated cytotoxicity The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay thapsigargin mediated cytotoxicity. (2914)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay IL-2 activated murine NK cell mediated cytotoxicity. (2977)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay IL -2 stimulated murine splenocyte mediated cytotoxicity. (2893)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay amyloid beta peptide mediated cytotoxicity. (2962)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay ATP mediated cytotoxicity. (2992)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay okadaic acid and calyculin mediated cytotoxicity. (2953)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay activated mouse neutrophil, macrophage and NK cell mediated cytotoxicity. (2973)

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess LDH release from primary cortical neurons following 24hr exposure to glutamate. Various compounds including 2.5S Nerve Growth Factor were assessed for their ability to block the glutamate-induced cytotoxicity. One of the neuroprotective factors, estrogen, was also examined for induction protein tyrosine kinase activity with the SignaTECT® Protein Tyrosine Kinase Assay System. The induction of PTK activity peaked at 1min and returned to normal within 10min. (0367)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to determine cytotoxicity of J774A.1 macrophages infected with various M. bovis bacille Calmette-Guérin constructs or L. monocytogenes. (1042)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay adenovirus mediated cytotoxicity. (2959)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay perforin and granulysin mediated cytotoxicity. (2941)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay System was used to monitor LDH release from T84 intestinal epithelial cells when treated with IFNgamma. (0256)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay Eschericia and Shigella mediated cytotoxicity. (2938)

Notes: Cytokine-induced killer (CIK) cells were transfected with an IL-7 expression vector. Cell-mediated cytotoxicity of the CIK cells was tested against A-704 renal carcinoma cells, HT 144 melanoma cells and REH pre B cell leukemic cells using the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay System. In all cases, the transfected CIK cells had greater cytotoxicity to the targets than the mock-transfected or untransfected CIK cells. An MTT reduction assay was used to demonstrate that the transfection was not toxic to the CIK cells. (1740)

Notes: Cytokine-induced killer (CIK) cells were transfected with an IL-7 expression vector. Cell-mediated cytotoxicity of the CIK cells was tested against A-704 renal carcinoma cells, HT 144 melanoma cells and REH pre B cell leukemic cells using the CytoTox^® 96 Non-Radioactive Cytotoxicity Assay. (1156)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay indomethacin mediated cytotoxicity. (2865)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to assay IL -2, mitomycin C-treated murine T-cell mediated cytotoxicity. (2979)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay System was used to monitor the Salmonella-induced lysis of bovine alveolar macrophages. (0171)

Notes: The CytoTox 96^® Non-Radioactive Cytotoxicity Assay was used to judge the protective, anti-apoptotic activity of various substances to treatment of mouse N18 neuroblastoma cells with Sindbis virus. (1176)