Aliprantis, A.O., Yang, R.-B., Mark, M.R., Suggett, S., Devaux, B., Radolf, J.D., Klimpel, G.R., Godowski, P. and Zychlinsky, A.
Notes: 293 cells stably expressing the human toll-like receptor-2 were challenged with various agents and analyzed for apoptosis. One test was a visual inspection for blebbing and the other was quantitation by TUNEL staining, using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay), followed by FACS analysis. In another assay for THP-1 cell death, the CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to monitor LDH release in response to bacterial lipoproteins. (1508)