Notes: The ProtoBlot^® Immunoscreening System was used in conjunction with a monoclonal antibody, to screen a Lambda gt11 library. The ProtoBlot^® Immunoscreening System has been discontinued, however components of the system are still available (Cat.# S3771, S3761). (0319)

Notes: The extracellular domain of the receptor was expressed in vitro using Promega's Rabbit Reticulocyte Lysate in the presence or absence of the microsomes. The 60kDa extracellular domain is processed to a 70kDa glycoprotein in the presence of the membranes. The paper also shows a titration of the membranes in the translation reaction from 1-4µl per reaction. More protein is processed with increasing amounts of the microsomes. (1627)