Notes: Total RNAs were extracted from the DRG cells using the ReliaPrep™ RNA Cell MiniPrep System and reverse transcribed. (4733)

Notes: Rat neural stem cells were cultured in a 3D hydrogel for either 7 days or 14 days. The hydrogel was destroyed by pipeting and RNA was isolated from the cells with the ReliaPrep™ RNA Cell Miniprep System. The quantity of RNA was determined with the QuantiFluor® RNA Dye System prior to dye-based real-time amplification with the GoTaq® 1-Step RT-qPCR System. The levels of three targets were compared at the 7 day and 14 day time points. (4595)

Notes: Mouse Endothelial Fibroblast were isolated from transgenic mice and human LEDGF/p75 expression levels were examined by dye-based RT-qPCR. The total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. (4605)

Notes: Total RNA was isolated from treated and untreated MC3T3-E1 mouse cells with the ReliaPrep™ RNA Cell Miniprep System and analyzed with an Agilent bioanalyzer. The isolated total RNA was labeled with Cy®3 dye and hybridized to an Agilent mouse array. (4615)

Notes: RNA was isolated from mouse basophils following fluorescence-activated cell sorting and used in RT-qPCR analysis. (4443)

Notes: RNA was extracted from cultured A549 cells and used in RT-qPCR to analyze the effect of transfected siRNAs. (4444)

Notes: Total RNA was isolated from ~150,000 O. dentatum larvae with the ReliaPrep™ RNA Tissue Miniprep System. The expression levels of multiple genes were analyzed by probe-based RT-qPCR. (4629)