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Plant Mol. Biol. 89, 215-27. Lace plant ethylene receptors, AmERS1a and AmERS1c, regulate ethylene-induced programmed cell death during leaf morphogenesis. 2015

Rantong, G., Evans, R., and Gunawardena, A.H.

Notes: These authors analyzed four transcript levels in dying and non-dying programmed cell death (PCD) cells. The cells were isolated from 8 samples of leaf tissue each by laser capture microdissection,and total RNA isolated using the ReliaPrep™ RNA Cell Miniprep System prior to dye-based RT-qPCR. (4619)

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Cancer Lett. 356, 994-1006. Lobatin B inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation. 2015

Kiss, I., et al.

Notes: SR-786 NPM/ALK-positive human anaplastic large cell lymphoma cell line was treated with lobatin B, a plant-derived natural compound. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reversed transcribed into cDNA with the GoScript™ Reverse Transcription System. Levels of NPM/ALK and NF-κB expression was measured with the dye-based GoTaq® qPCR System. SR-786 cells were also analyzed for functioning metabolism with the CellTiter-Blue® Cell Viability Assay and apoptosis with the Apo-ONE™ Homogeneous Caspase-3/7 Assay. (4604)

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Biochem. Biophys. Res. Commun. 464, 603-10. Monoacylglycerol lipase promotes Fcγ receptor-mediated phagocytotsis in microglia but does not regulate LPS-induced upregulation of inflammatory cytokines. 2015

Kouchi, Z.

Notes: Total RNA from primary mouse microglia and BV-2 microglia cells for expression analysis of monoacylglycerol lipase and inflammatory cytokine levels via dye-based RT-qPCR. The RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. (4626)

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BMC Biotechnol. 15, 109. Novel avian single-chain fragment variable (scFv) target dietary gluten and related natural grain prolamins, toxic entities of celiac disease. 2015

Stadlmann, V., Harant, H., Korschineck, I., Hermann, M., Forster, F. and Missbichler, A.

Notes: Chickens were immunized with PT-gliadin and splenocytes were isolated. Anti-PT-gliadin-positive cells were enriched on magnetic beads coated with PT-gliadin. Total RNA was extracted from the bead-bound cells with the ReliaPrep™ RNA Cell Miniprep System and used to clone the chicken IgY hypervariable regions. The scFv expression vectors were purified with the PureYield™ Plasmid Miniprep or Midiprep System prior to transformation into BL21(DE3) cells. ELISA assays were developed with standard TMB substrate and read with the GloMax® Instrument. (4618)

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Tumor Biol. , 1-12 DOI: 10.1007/s13277-015-3781-8. Phenylbutyrate–a pan-HCAD inhibitor–suppressed proliferation of glioblastoma LN-229 cell line. 2015

Kusaczuk, M., Krętowski, R., Bartoszewicz, M., Cechowska-Pasko, M.

Notes: The potency of the phenylbutyrate (PBA) HDAC inhibitor was assayed on LN-229 and LN-18 human glioblastoma cells using the HDAC-Glo™ I/II Assay. LN-229 cells were treated for 24 or 48 hours with PBA and analyzed for changes in expression of seven targets via dye-based RT-qPCR. The total RNA for these studies was isolated with the ReliaPrep™ RNA Cell Miniprep System. The total RNA was analyzed with an Agilent Bioanalyzer and samples with RIN >9 were chosen for the RT-qPCR analysis. (4599)

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Mediators Inflammation , Article ID: 737310. Role of calprotectin as a modulator of the IL27-mediated proinflammatory effect on endothelial cells. 2015

Dorosz, S.A., Ginolhac, A., Kähne, T., Naumann, M., Sauter, T., Salsmann, A., and Bueb, J.-L.

Notes: Human Umbilical Vein Endothelial cells (HUVECs) were treated with IL-27, calprotectin, TNFα or combinations. Cell number per well was controlled by lysing the cells and measuring the LDH release with the CytoTox 96® Assay. Changes in gene expression were monitored by probe-based qPCR with cDNA made with the GoScript™ Reverse Transcription System using total RNA isolated using the ReliaPrep™ RNA Cell Miniprep System. Changes in protein levels upon treatment were evaluated by mass spec using Trypsin Gold for peptide analysis. (4598)

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Antiviral Res. 124, 69–76. The non-pathogenic Henipavirus Cedar paramyxovirus phophoprotein has a compromised ability to target STAT1 and STAT2. 2015

Lieu, K.G., Marsh, G.A., Wang, L.-F. and Netter, H.J.

Notes: HEK 293 cells were transfected with various viral protein expression vectors and expression levels measured in the presence or absence of interferon-α using RT-qPCR. Total RNA was isolated from the cells using the ReliaPrep™ RNA Cell Miniprep System and converted to cDNA with the GoScript™ Reverse Transcription System prior to dye-based qPCR. (4613)

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Forensic Sci. Int. Genet. 17, 35-42. Transcriptomic analysis of degraded forensic body fluids. 2015

Lin, M.-H., Jones, D.F. and Fleming, R.

Notes: Bodily fluids (buccal, circulatory blood, vaginal material, menstrual blood) were collected on sterile Cultiplast® rayon swabs and aged for zero, two or six weeks. Total RNA was extracted with the ReliaPrep™ RNA Cell Miniprep System and eluted in 50µl. Absence of detectable levels of human DNA (due to the on-column DNase treatment) was confirmed with a qPCR-based human forensic DNA quantitation test. RNA was analyzed with the Agilent Bioanalyzer, then prepped for RNAseq analysis following rRNA depletion. (4625)

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Genes Immun. 15, 33-7. A comprehensive analysis of the binding of anti-KIR antibodies to activating KIRs. 2014

Czaja, K., Borer, A.-S., Schmied, L., Terszowski, G., Stern, M., and Gonzalez, A.

Notes: Peripheral blood mononuclear cells were labeled with antibodies and sorted. Each sorted subset was immediately processed for total RNA with the ReliaPrep™ RNA Cell Miniprep System. The RNA was converted to cDNA then analyzed for expression level of three targets by qPCR with the dye-based GoTaq® qPCR System. (4600)

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PLos ONE 9, e106190. Anoctamin-1 in the juvenile rat urinary bladder. 2014

Bijos, D.A., Drake, M.J., and Vahabi, B.

Notes: Total RNA was extracted from mucosa and denuded-detrusor layers of rat bladders with the ReliaPrep™ RNA Tissue Miniprep System and converted to cDNA using GoScript™ Reverse Transcription System prior to PCR amplification. (4608)

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Toxicol. Rep. 1, 589-95. Arsenic (+3 oxidation state) methyltransferase is a specific but replaceable factor against arsenic toxicity. 2014

Tokumoto, M., Kutsukake, N., Yamanishi, E., Katsuta, D., Anan, Y., and Ogra, Y.

Notes: Total RNA was isolated from untreated HepG2 cells to clone the AS3MT transcript by RT-PCR, and for RT-qPCR analysis following AS3MT-directed siRNA treatment. The total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. The health of the siRNA-treated cells was monitored with the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (4620)

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Mol. Cell. Biol. 34, 3486–99. Cellular migration and invasion uncoupled: Increased migration is not an inexorable consequence of epithelial-to-mesenchymal transition. 2014

Schaeffer, D., Somarelli, J.A., Hanna, G. and Palmer, G.M.

Notes: Rat renal DT and A3 cells were examined for differences in EGFR expression by RT-qPCR. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reverse transcribed with the GoScript™ Reverse Transcription System followed by dye-based qPCR analysis. (4617)

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Mol. Cell. Biol. 34, 3486 –3499. Cellular Migration and Invasion Uncoupled: Increased Migration Is Not an Inexorable Consequence of Epithelial-to-Mesenchymal Transition 2014

Schaeffer, D., Somarelli, J.A., Hanna, G., Palmer, G.M. and Garcia-Blanco, M.A.

Notes: Total RNA was isolated using the ReliaPrep™ RNA cell Miniprep System. The Improm-II™ Reverse Transcription System was used to synthesize random hexamer-primed cDNA from 1g of total RNA. (4738)

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J. Virol. 88, 8998-9009. Crucial Roles of Interleukin-7 in the Development of T Follicular Helper Cells and in the Induction of Humoral Immunity 2014

Seo, Y.B., Im, S. J., Namkoong, H., Kim, S.W., Choi, Y.W., Kang, M.C., Lim, H.S., Jin, H.T., Yang, S.H., Cho, M.L., Kim, Y.-M., Lee, S.-W., Choi, Y.K., Surh, C.D. and Sunga, Y.C.

Notes: <p>RNA was prepared using a ReliaPrep™ RNA Cell Miniprep System and reverse transcribed. (4734)

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PLos ONE 9, e107236. Familly with sequence similarity 5, member C (FAM5C) increases leukocyte adhesion molecules in vascular endothelial cells: Implication in vascular inflammation. 2014

Sato, J., Kinugasa, M., Satomi-Kobayashi, S., Hatakeyama, K., Knox, A.J., Asada, Y., Wierman, M.E., Hirata, K., and Rikitake, Y.

Notes: Human Umbillical Vein Endothelial Cells (HUVECs) were transfected with FAM5C or mock transfected and tested for changes in specific gene expression and changes associated with TNFα treatment. RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System prior to RT-qPCR with a dye-based system. Mice were also treated with TNFα and expression levels were examined for specific targets using total RNA isolated from brain and aorta using the ReliaPrep™ RNA Tissue Miniprep System followed by dye-based RT-qPCR. (4597)

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Mol. Metab. 3, 731-41. Fractalkine (CX3CL1), a new factor protecting β-cells against TNFα. 2014

Rutti, S., Arous, C., Schvartz, D., Timper, K., Sanchez, J.-C., Dermitzakis, E., Donath, M.Y., Halban, P.A., and Bousakri, K.

Notes: Human pancreas islet cells were isolated by cell sorting and total RNA was isolated from 100 islet equivalents using the ReliaPrep™ RNA Cell Miniprep System. The RNA was analyzed by probe-based RT-qPCR. (4622)

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PLos ONE 9, e98877. Induction of cell-mediated immune responses in mice by DNA vaccines that express hepatitis C virus NS3 mutants lacking serine protease and NTPase/RNA helicase activities. 2014

Ratnoglik, S.L., Jiang, D.-P., Aoki, C., Sudarmono, P., Shoji, I., Deng, L. and Hotta, H.

Notes: Primary splenocytes were isolated from mice immunized with an NS3 expression vector and examined for IFN-γ expression by RT-qPCR. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and converted to cDNA with the GoScript™ Reverse Transcription System. The affect of wildtype and mutant NS3 on interferon-β promoter activity with an IFN-β promoter firefly luciferase vector and pRL-TK vector control was measured with the Dual-Luciferase® Reporter Assay System. LDH release was measured with the CytoTox 96® Cytotoxicity Assay in a cytotoxic T-lymphocyte assay using cultured splenocytes from the immunized mice. (4609)

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Fitoterapia. 99, 276–83. Inhibition of hepatitus C virus replication by chalepin and pseudane IX isolated from Ruta angustifolia leaves. 2014

Wahyuni, T.S., Widyawaruyanti, A., Lusida, M.I., Fuad, A., Soetjipto, Fuchino, H., Kawahara, N., Hayashi, Y., Aoki, C. and Hotta, H.

Notes: Huh7.5 cells were infected with HCV and treated with the plant-derived chemicals. The expression of viral messages was analyzed by RT-qPCR. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reverse transcribed with the GoScript™ Reverse Transcription System before dye-based qPCR analysis. (4616)

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Cardiovasc Res. 103, 607–18. Mechanisms underlying capsaicin effects in canine coronary artery: implications for coronary spasm. 2014

Hiett, S.C., Owen, M.K., Li, W., Chen, X., Riley, A., Noblet, J., Flores, S., Sturek, M., Tune, J.D. and Obukhov, A.G.

Notes: Total RNA was isolated from freshly collected, snap-frozen canine coronary arteries using the ReliaPrep™ RNA Tissue Miniprep System and used for semi-quantitative RT-PCR with limited cycle numbers. (4614)

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Cardiovasc Res. 103, 607-618. Mechanisms underlying capsaicin effects in canine coronary artery: Implications for coronary spasm 2014

Hiett, S. C., Owen, M. K., Li, W., Chen, X., Riley, A., Noblet, J., Flores, S., Sturek, M., Tune, J. D. and Obukhov, A. G.

Notes: Total RNA was isolated from freshly collected and snap-frozen canine coronary arteries using the ReliaPrep™ RNA Tissue Miniprep System. (4724)

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EMBO J. 33, 1565-1581. MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures 2014

Muraoka, N., Yamakawa, H., Miyamoto, K., Sadahiro, T., Umei, T., Isomi, M., Nakashima, H., Akiyama, M., Wada, R., Inagawa, K., Nishiyama, T., Kaneda, R., Fukuda, T., Takeda, S., Tohyama, S., Hashimoto, H., Kawamura, Y., Goshima, N., Aeba, R., Yamagishi, H., Fukuda, K. and Ieda, M.

Notes: For construction of the Snai1 30 UTR reporter, the CMV promoter was subcloned into the promoterless pGL3-Basic vector upstream of the luciferase gene. A 755-bp Snai1 30 UTR fragment containing miR-133a-binding sites was amplified by PCR and subcloned into the modified pGL3-Basic vector. The activities of firefly luciferase and renilla luciferase in the control vector were determined by the Dual-Glo® Luciferase Assay System. RNA was extracted from MEFs, GMT-, GMT/miR-133-, or GMT/miR-133/ Snai1-induced aMHC-GFP+ cells, neonatal mouse heart tissues, HCFs, GMTMM-, GMTMM/miR-133-, GMTMM/miR-133/Snai1-transduced HCFs using ReliaPrep™ RNA Cell Miniprep System for gene microarray analysis. (4737)

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Biochem. Biophys. Res. Commun. 446, 30-6. Reversine increases the plasticity of lineage-committed preadipocytes to osteogenesis by inhibiting adipogenesis through induction of TGF-β pathway in vitro. 2014

Park, J.G., Lee, D.-H., Moon, Y.S., and Kim, K.-H.

Notes: 3T3-L1 preadipocytes were treated with various levels of reversine and monitored for induction of apoptosis with the Caspase-Glo® 3/7 Assay with data collected on a GloMax® Instrument. Changes in gene expression of three targets upon reversine treatment were examined through total RNA isolation with the ReliaPrep™ RNA Cell Miniprep System, reverse transcription with the ImProm-II™ Reverse Transcription System followed by dye-based qPCR analysis. (4596)

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Acta Biomater. 10, 3177-87. Three-dimensional hydrogel scaffolds facilitate in vitro self-renewal of human skin-derived precursors. 2014

Wang, X., Liu, S., Zhao, Q., Li, N., Zhang, H., Zhang, X., Lei, X., Zhao, H., Deng, Z., Qiao, J., Cao, Y., Ning, L., Liu, S., and Duan, E.

Notes: Human Skin Progenitor (HSK) cells were cultured on hydrogels and either left alone or differentiated into various skin cell types. Gene expression changes were monitored by isolating total RNA from collagenase and hyaluronidase treated hydrogels with the ReliaPrep™ RNA Cell Miniprep System followed by cDNA synthesis with the GoScript™ Reverse Transcription System and qPCR with the dye-based GoTaq® qPCR System. (4602)

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FASEB J. 28, 946–55. Transcriptional control of the B3GALT5 gene by a retroviral promoter and methylation of distant regulatory elements. 2014

Zulueta, A., Caretti, A., Signorelli, P., Dall'olio, F. and Trinchera, M.

Notes: RNA was isolated from several cell lines using the SV Total RNA Isolation System and ReliaPrep™ RNA Cell Miniprep System. RNA was used in competitive RT-PCR to characterize splice variants of the B3GALT5 long terminal repeat, HNFα and HNFβ1. (4445)

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PLos ONE 8, e84071. Gene expression profiling of early hepatic stellate cell activation reveals a role for Igfbp3 in cell migration. 2013

Mannaerts, I., Schroyen, B., Verhulst, S., Van Lommel, L., Schuit, F., Nyssen, M. and van Grunsven, L.A.

Notes: Mouse hepatic stellate cells (HSCs) were cultured with valproic acid for various times. Total RNA was isolated and analyzed for multiple transcripts by RT-qPCR using the dye-based GoTaq® RT-qPCR System. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and used for RT-qPCR and expression profiling with an Affymetrix genechip array. (4610)

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