We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Limited Functionality Warning

We are currently updating our network. During this time, certain functionality may be unavailable, including online orders. We apologize for any inconvenience this may cause you.

Please contact Customer Service with any questions or comments.
Phone: (608) 274-4330
Toll-Free Phone: (800) 356-9526
Email: custserv@promega.com
Hours: 7am – 6pm, CST, Monday-Friday

Citations Search

Sort By:

Int. J. Nanomedicine 13, 2279–2294. Silica nanoparticle-induced oxidative stress and mitochondrial damage is followed by activation of intrinsic apoptosis pathway in glioblastoma cells.  2018

Kusaczuk, M., Krętowski, R., Naumowicz, M., Stypułkowska, A., and Cechowska-Pasko, M.

Notes: These authors investigated silicon dioxide nanoparticles (SiNPs) as a potential cancer treatment and identified the mechanism mediating cancer cell death. The Caspase-Glo® 3/7, Caspase-Glo® 9, CellTiter-Glo® luminescent cell-viability, ROS-Glo™, and Caspase-Glo® 1 assays were used to monitor cellular response to SiNPs. Together, SiNPs were identified to activate the intrinsic apoptosis pathway and lead to cell death.

  (5165)

Expand Full Notes »

Stem Cells 36, 337–348. Differences in the Activity of Endogenous Bone Morphogenetic Protein Signaling Impact on the Ability of Induced Pluripotent Stem Cells to Differentiate to Corneal Epithelial‐Like Cells 2017

Kamarudin, T.A., Bojic, S., Collin, J., Yu, M., Alharthi, S., Buck, H., Shortt, A., Armstrong, L., Figueiredo, F.C., Lako, M.

Notes: RNA was extracted from the cells collected from differentiating hESC and hiPSC at days 0, 9, and 20 using the ReliaPrep RNA Cell Miniprep System. The RNA quality was evaluated using the Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, MA). One microgram of extracted RNA was converted into cDNA using reverse transcription (GoScript Transcription System). Quantitative real‐time polymerase chain reaction (qRT‐PCR) was carried out using the QuantStudio 7 Flex Real‐Time PCR System (Thermo Fisher Scientific, MA) and GoTaq qPCR Master Mix. pGL3 BRE Luciferase was used for plasmid lipofection to transfect the cells in each well of a 24-well plate. Cells that were transfected with empty vector (pGL3‐Basic) or BMP reporter (pGL3 BRE Luciferase) were cotransfected with empty Renilla vector (pRL‐Null). Luciferase activities were evaluated with a Dual‐Luciferase Assay System. (4982)

Expand Full Notes »

Biochem. Biophys. Res. Commun. 489, 248-254. Exosomes isolated from sera of mice fed Lactobacillus strains affect inflammatory cytokine production in macrophages in vitro 2017

Aoki-Yoshida, A., Saito, S., Tsuruta, T., Ohsumi, A., Tsunoda, H., Sonoyama, K.

Notes: ReliaPrep RNA Cell Miniprep System was used to isolate Total RNA from peritoneal exudate cells of probiotics. Then the RNA was reverse transcribed to generate first-strand cDNA and RT-qPCR was performed using non-promega products. (4981)

Expand Full Notes »

PLos ONE 12, e0171431. Potential roles of DNA methylation in the initiation and establishment of replicative senescencerevealed by array-based methylome and transcriptome analyses. 2017

Sakaki, M., Ebihara, Y., Okamura, K., Nakabayashi, K., Igarashi, A., Matsumoto, K., Hata, K., Kobayashi, Y. and Maehara, K.

Notes: Total RNA was isolated from normal human diploid fibroblast TIG-3 cells with a ReliaPrep RNA Cell MiniPrep System. The isolated RNA was used to synthesize cRNA which was then purified using non-Promega products. Total RNA including miRNA was isolated with a ReliaPrep miRNA Cell and Tissue MiniPrep System. (4980)

Expand Full Notes »

Mol. Cell. Biol. 36, 1297-1309. Gtf2ird1-Dependent Mohawk Expression Regulates Mechanosensing Properties of the Tendon 2016

Kayama, T., Mori, M., Ito, Y., Matsushima, T., Nakamichi, R., Suzuki, H., Ichinose, S., Saito, M., Marumo, K. and Asahara, H.

Notes: RNA collection from rat Achilles tendon was performed using a ReliaPrep™ RNA Tissue Miniprep System. RNA collection from cells was performed using a ReliaPrep™ RNA Cell Miniprep System. (4735)

Expand Full Notes »

FASEB J. 30(2), 24-34. Cold-inducible RBM3 inhibits PERK phosphorylation through cooperation with NF90 to protect cells from endoplasmic reticulum stress. 2016

Zhu, X., Zelmer, A., Kapfhammer, J.P., and Wellmann, S.

Notes: HEK 293 cells were treated with thapsigargin and expression of target mRNAs was examined. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and reverse transcribed with the GoScript™ Reverse Transcription System followed by quantitation with the dye-based GoTaq® qPCR System. (4606)

Expand Full Notes »

Forensic Sci. Int. Genet. 20, 61-70. Degraded RNA transcript stable regions (StaRs) as targets for enhanced forensic RNA body fluid identification. 2016

Lin, M.-H., Albani, P.P. and Fleming, R

Notes: Bodily fluids (buccal, circulatory blood, vaginal material, menstrual blood) were collected on sterile Cultiplast® rayon swabs. Total RNA was extracted from each sample with the ReliaPrep® RNA Cell Miniprep System and eluted in 50µl. Absence of detectable levels of human DNA (due to the on-column DNase treatment) was confirmed with a qPCR-based human forensic DNA quantitation test. Total RNA was converted to cDNA then amplified in a multiplex reaction with target-specific primer for analysis by capillary electrophoresis. (4601)

Expand Full Notes »

Microbiol Immunol. 60, 17-25. Interaction of the hepatitis B virus X protein with the lysine methyltransferase SET and MYND domain-containing 3 induces activator protein 1 activation. 2016

Hyashi, M., Deng, L., Chen, M., Gan, X., Shinozaki, K., Shoji, I., and Hotta, H.

Notes: The effect of SMYD3 and ERK2 on hepatitis B RNA expression was examine in transfected Huh-7.5 human hepatoma cells. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and converted to cDNA with the GoScript™ Reverse Transcription System followed by dye-based qPCR analysis. The effect of SMYD3 and ERK2 on AP1-dependent and NF-κB-dependent gene expression in the cells was examined with the pGL4.44 [luc2P/AP1 RE/Hygro] and pGL4.32 [luc2P/NF-κB-RE/Hygro] Reporter vectors using the Dual-Luciferase® Reporter Assay System with a pRL-TK Renilla luciferase control. Reporter assays were read with a GloMax® Instrument. (4603)

Expand Full Notes »

J. Biol. Chem. jbc.M115.707174, epub. Megakaryocytic maturation in response to shear flow is mediated by the activator protein 1 (AP-1) transcription factor via mitogen-activated protein kinase (MAPK) mechanotransduction. 2016

Luff, S.A., and Papoutsakis, E.T.

Notes: RNA from unstressed and shear-stressed human megakaryocytes was isolated with the ReliaPrep™ RNA Cell Miniprep System. The isolated RNA was ran through a Bioanalyzer and RIN values >7 were chosen for further study with Agilent human gene expression arrays. (4631)

Expand Full Notes »

J. Biol. Chem. 291, 7831–7843. Megakaryocytic Maturation in Response to Shear Flow Is Mediated by the Activator Protein 1 (AP-1) Transcription Factor via Mitogen-activated Protein Kinase (MAPK) Mechanotransduction 2016

Luff, S.A. and Papoutsakis, E.T.

Notes: RNA from three biological replicates of unstressed (static) and shear-stressed (flow) megakaryocytes were isolated using the ReliaPrep™ RNA Cell Miniprep System. (4736)

Expand Full Notes »

Neuroscience 314, 90-105. Role of brain aldosterone and mineralcorticoid receptors in aldosterone-salt hypertension in rats. 2016

Wang, H.-W., Huang, B.S., Chen, A., Ahmad, M., White, R.A.,and Leenen, F.H.H.

Notes: Serial 50µm thick coronal rat brain slices were cryosectioned and specific areas of interest were identified and micro-punched. Total RNA was extracted from the micro-punches with the ReliaPrep™ RNA Tissue Miniprep System. The RNA was used for dye-based, two-step RT-qPCR. (4593)

Expand Full Notes »

Proc. Natl. Acad. Sci. USA 113, E3492-3500. Transplantation of iPSC-derived TM cells rescues glaucoma phenotypes in vivo 2016

Zhu, W., Gramlich, O.W., Laboissonniere, L., Jain, A., Sheffield, V.C., Trimarchi, J.M., Tucker, B.A. and Kuehn, M.H.

Notes: For global transcriptional analysis of mouse anterior segments, tissue was harvested from treated and control eyes 12 weeks after transplantation. Immediately after enucleation the trabecular meshwork was excised, preserved in RNAlater, and stored at −80 °C until further processing. RNA was then isolated using the ReliaPrep™ RNA Cell Miniprep System. (4739)

Expand Full Notes »

Nucl. Acids Res. epub ahead of print. Defining the essential function of FBP/KSRP proteins: Drosophila Psi interacts with the mediator complex to modulate MYC transcription and tissue growth 2016

Guo, L., Zaysteva, O., Nie, Z., Mitchell, N.C., Lee, A.J.E., Ware, T., Parsons, L., Luwor, R., Poortinga, G., Hannan, R.D., Levens, D.L. and Quinn, L.M.

Notes: The authors used the ReliaPrep RNA Cell Miniprep System to isolate RNA from Drosophila wing imaginal discs prior to cDNA synthesis. (4727)

Expand Full Notes »

mBio. 7, e01865-15. Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication 2016

Phillips, S.L., Soderblom, E.J., Bradrick, S.S. and Garcia-Blanco, M.A.

Notes: Total RNA was extracted using the ReliaPrep™ RNA Cell MiniPrep System for RT-qPCR. (4731)

Expand Full Notes »

Genetics 203, 733–748. Nuclear envelope retention of LINC Complexes is promoted by SUN-1 oligomerization in the Caenorhabditis elegans germ line 2016

Daryabeigi, A., Woglar, A., Baudrimont, A, Silva, N., Paouneskou, D., Vesely, C., Rauter, M., Penkner, A., Jantsch, M. and Jantsch, V.

Notes: ReliaPrep™ RNA Tissue Miniprep columns were used to clean up RNA extracted from C. elegans hermaphrodites prior to cDNA synthesis reactions. (4720)

Expand Full Notes »

Cell Mol. Neurobiol. 35, 1137-47. 6-OHDA-induced changes in Parkinson’s disease-related gene expression are not affected by the overexpression of PGAM5 in in vitro differentiated embryonic mesencephalic cells. 2015

Stępkowski, T.M., Wasyk, I., Grzelak, A., and Kruszewski, M.

Notes: LUHMES, a human embryonic mesencephalic cell line, was tested for the impact of overexpression of the PGAM5, a mitochondrial protein implicated in the molecular processes of Parkinson’s disease, with regard to 84 Parkinson’s associated genes. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and quality determined with a Agilent Bioanalyzer. The RNA was converted to cDNA and analyzed with the a Parkinson’s disease PCR array. The cDNA was also used in probe-based qPCR reactions. (4627)

Expand Full Notes »

Nat. Commun. 6, 7888. Actin nucleation by WH2 domians at the autophagosome.

.
2015

Coutts, A.S., and La Thangue, N.B.

Notes: Total RNA was isolated from U2OS treated with either SAHA or grown under starvation conditions and the levels of two transcripts were monitored by dye-based RT-qPCR. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. (4628)

Expand Full Notes »

Oncol. Lett. 9, 330-4. Alternative splicing isoform in succinate dehydrogenase complex, subunit C causes downregulation of succinate-coenzyme Q oxidoreductase activity in mitochondria. 2015

Satoh, N., Yokoyama, C., Itamura, N., Miyajima-Nakano, Y., and Hisatomi, H.

Notes: Total RNA was isolated from HTC-15 human colorectal carcinoma cells with the ReliaPrep™ RNA Cell Miniprep System. Isolated RNA was used in an RT-PCR method to amplify alternative splice variants of the target transcript. Once identified, a RT-qPCR experiment was designed to look for the major splice variants in normal cell lines derived from 15 human tissues (RNA isolation details not provided, assumed to be the same as HTC-15). The alternative splice variants were overexpressed in HTC-15 cells by transfection via FuGENE® HD Transfection Reagent and noted a decline in full-length expression. (4607)

Expand Full Notes »

FASEB J. 29, 394–407. Autophagy modulates amino acid signaling network in myotubes: differential effects on mTORC1 pathway and the integrated stress response. 2015

Yu, X. and Long, Y.C.

Notes: C2C12 myotubes treated with BaF with and without amino acid deprivation were analyzed for gene expression by RT-qPCR. The RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System and converted to cDNA with the ImProm-II™ Reverse Transcription System followed by dye-based qPCR. (4611)

Expand Full Notes »

BMC Cancer 15, 634. Chemotherapy induces Notch1-dependent MRP1 up-regulation, inhibition of which sensitizes breast cancer cells to chemotherapy. 2015

Kim, B., Stephen, S.L., Hanby, A.M., Horgan, K., Perry, S.L., Richardson, J., Roundhill, E.A., Valleley, E.M.A., Verghese, E.T., Williams, B.J., Thorne, J.L. and Hughes, T.A.

Notes: T47D and HB2 breast cancer cell lines were treated with doxorubicin and expressed in three target genes. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System, reverse transcribed with the GoScript™ Reverse Transcription System and analyzed with the dye-based GoTaq® qPCR System. (4612)

Expand Full Notes »

Biochem. Biophys. Res. Commun. 461, 35-41. Downregulation of the DNA repair enzyme apurinic/apyrimidinic endonuclease 1 stimulates transforming growth factor-β1 production and promotes actin rearrangement. 2015

Sakai, Y., Yamamori, T., Hironobu, Y., and Osamu, I.

Notes: Total RNA was isolated from HeLa and A549 cells following downregulation of APE1 via siRNA transfection using the ReliaPrep™ RNA Cell Miniprep System. The levels of mRNA for a number of genes were monitored through dye-based RT-qPCR at 48, 72 and 96 hours post siRNA transfection. (4591)

Expand Full Notes »

Biochem. Biophys. Res. Commun. 463, 1218-24. Establishment and culture optimization of a new type of pituitary immortalized cell line. 2015

Kokubu, Y., Asashima, M., and Kurisaki, A.

Notes: Human pituitary cells were immortalized through expression of three genes. The immortalized cells were induced to secrete hormones and gene expression was monitored by RT-qPCR. Total RNA was extracted with the ReliaPrep™ RNA Cell Miniprep System. (4623)

Expand Full Notes »

World J. Gastroenterol. 21, 3976-87. Fucosylation is a common glycosylation type in pancreatic cancer stem cell-like phenotypes. 2015

Terao, N., Takamatsu, S., Minehira, T., Sobajima, T., Nakayama, K., Kamada, Y., and Miyoshi, E.

Notes: Panc-1 pancreatc cancer cells were made resistant to a drug over time. The glycan structures on the surface of resistant and sensitive cells by passage over a lectin array, identifying a difference in fucosylation. Sensitive and resistant cell total RNA was isolated and examined by RT-qPCR for genes related to fucosylation. Total RNA was isolated with the ReliaPrep™ RNA Cell Miniprep System. (4624)

Expand Full Notes »

Mol. Metab. 5, 122-31. IL-13 improves beta-cell survival and protects against IL-1beta-induced beta-cell death. 2015

Rütti, S., Howald, C., Arous, C., Dermitzakis, E., Halban, P.A., and Bouzakri, K.

Notes: Primary rat beta-cells were prepared from islets and sorted by flow cytometry. Total RNA was prepared with the ReliaPrep™ RNA Cell Miniprep System prior to RNAseq. (4621)

Expand Full Notes »

Dev. Comp.Immunol. 52, 75-80. Kinetics of lymphocyte subpopulation in allogeneic grafted scales of ginbuna crucian carp. 2015

Shibasaki, Y., Matsuura, Y., Toda, H., Imabayashi, N., Nishino, T., Uzumaki, K., Hatanaka, C., Yabu, T., Moritomo, T., and Nakanishi, T.

Notes: Total RNA was extracted from grafted scales and surrounding epidermis of recipient fish with the ReliaPrep™ RNA Tissue Miniprep System. The RNA was used for dye-based two-step RT-qPCR to examine 12 transcript levels. (4630)

Expand Full Notes »