Notes: Studies were performed in H4IIE rat hepatoma cells. The pGL3-based Vectors were co-transfected with the Renilla control vector (pRL-SV40 Vector) at a 6:1 ratio and assayed using the Dual-Luciferase^® Reporter Assay System. (1489)

Notes: Luciferase studies were performed in NIH3T3 cells expressing 20,000 muscarinic acetylcholine receptors per cell using constructs prepared in the pGL3 Promoter Vector. (1276)

Notes: Studies were performed in the neuroblastoma cells lines SY5Y and SK-N-BE and the human rhabdomyosarcoma cell line TE671. All reporter activities were reported as fold-stimulation over pGL3 Basic Vector basal expression. (1570)

Notes: The Dual-Luciferase^® Reporter Assay System was used to study transfections of IMR32 and NIH3T3 cells with firefly luciferase vector (pGL3 Basic) constructs. Transfections were controlled with co-transfected pRL-CMV Vector. Tth DNA Polymerase was used as a high temperature reverse transcriptase. SP6 RNA Polymerase was used to produce probes for RNase protection assays. (0558)

Notes: Luciferase studies were performed in SV40 T type II cells and IMR90 fibroblasts using constructs prepared in the pGL3 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. The hepatocyte nuclear factor 3beta was produced in vitro with the TNT^® Coupled Reticulocyte Lysate System and used for gel shift analysis. (0495)

Notes: Reporter studies were performed in THP-1 monocytic cells, Jurkat T-cell leukemic cells and K562 myelogenous leukemia cells. Experimental promoter constructs were prepared in the pGL3 Basic Vector. Transfections were control by cotransfection with the pRL-CMV Vector, a source of Renilla luciferase. The activity of the two luciferases was determined with the Dual-Luciferase® Reporter Assay System. Ratios of pGL3-based Vectors to the pRL-CMV Vector were not reported but 0.5µg of the pRL-CMV Vector was used in each transfection. (0630)

Notes: Promega's Luciferase Assay System and pGL3 Basic Vector were used to study gene expression in primary rat pinealocytes. (1455)

Notes: Luciferase studies were performed in HOS D4 osteosarcoma cells. Reporter constructs were prepared in the pGL2 Enhancer Vector. (0686)

Notes: Luciferase studies were performed in PAM 212 mouse keratinocyte cells and NIH3T3 cells. They cloned a 14kb promoter fragment into the pGL3-Basic Vector and used this and the pGL3-Control Vector for their studies. (0303)

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard^® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)

Notes: Expression of luciferase reporter constructs, derived from the pGL3 Promoter Vector, was studied in 3T3 cells. (0165)

Notes: CHO-K1 cells were transfected at ~80% confluency with four plasmids: (1) a CMV-driven vector expressing the wildtype or mutant human insulin receptor; (2) a pGL3-Basic-derived Vector containing five GAL4 binding sites upstream of the firefly luciferase reporter; (3) the pRL-CMV Control Vector; and (4) a fusion of the GAL4-binding domain and Elk-1 transcription factor. The Dual-Luciferase^® Reporter Assay System was used to confirm that specific mutations in the insulin receptor negate its ability to activate the Elk-1 transcription factor. (0896)

Notes: pGL3-Control Vector and the Luciferase Assay System were used for in vivo studies on murine quadricep muscles by injection of DNA. Extracts for assaying the tissues were prepared using the CCLR. (1066)

Notes: The Tfx™-50 Reagent was used to transfect pGL3 Vectors into NIH3T3 cells as well as microinjection into amniotic fluid (clone A31). (1243)