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J. Biol. Chem. 272, 31755-31763. Signal transduction-mediated activation of the aryl hydrocarbon receptor in rat hepatoma H4IIE cells. 1997

Backlund, M., Johansson, I., Mkrtchian, S., Ingelman-Sundberg, M.

Notes: Studies were performed in H4IIE rat hepatoma cells. The pGL3-based Vectors were co-transfected with the Renilla control vector (pRL-SV40 Vector) at a 6:1 ratio and assayed using the Dual-Luciferase® Reporter Assay System. (1489)

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J. Biol. Chem. 272, 20691-20697.. Signaling from G protein-coupled receptors to the c-jun promoter involves the MEF2 transcription factor. Evidence for a novel c-jun amino-terminal kinase-independent pathway 1997

Coso, O. A. , Montaner, S. , Fromm, C. , Lacal, J. C. , Prywes, R. , Teramoto, H. , Gutkind, J. S.

Notes: Luciferase studies were performed in NIH3T3 cells expressing 20,000 muscarinic acetylcholine receptors per cell using constructs prepared in the pGL3 Promoter Vector. (1276)

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Mol. Pharmacol. 51, 250-261. Structural and functional characterization of the human alpha3 nicotinic subunit gene promoter. 1997

Fornasari, D., Battaglioli, E., Flora, A., Terzano, S. and Clementi, F.

Notes: Studies were performed in the neuroblastoma cells lines SY5Y and SK-N-BE and the human rhabdomyosarcoma cell line TE671. All reporter activities were reported as fold-stimulation over pGL3 Basic Vector basal expression. (1570)

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J. Biol. Chem. 272, 17112-17117. Structure of the m1 muscarinic acetylcholine receptor gene and its promoter 1997

Pepitoni, S., Wood, I.C. Buckley, N.J.

Notes: The Dual-Luciferase® Reporter Assay System was used to study transfections of IMR32 and NIH3T3 cells with firefly luciferase vector (pGL3 Basic) constructs. Transfections were controlled with co-transfected pRL-CMV Vector. Tth DNA Polymerase was used as a high temperature reverse transcriptase. SP6 RNA Polymerase was used to produce probes for RNase protection assays. (0558)

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J. Biol. Chem. 272, 26285-26294. TGT3, thyroid transcription factor I, and Sp1 elements regulate transcriptional activity of the 1.3-kilobase pair promoter of t1alpha, a lung alveolar type I cell gene 1997

Ramirez, M.I., Rishi, A.K., Cao, Y.X., Williams, M.C.

Notes: Luciferase studies were performed in SV40 T type II cells and IMR90 fibroblasts using constructs prepared in the pGL3 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. The hepatocyte nuclear factor 3beta was produced in vitro with the TNT® Coupled Reticulocyte Lysate System and used for gel shift analysis. (0495)

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J. Biol. Chem. 272, 30662-30671. The Human CC Chemokine Receptor 5 (CCR5) Gene. Multiple transcripts with 5'-end heterogeneity, dual promoter usage, and evidence for polymorphisms within the regulatory regions and noncoding exons 1997

Mummidi, S., Ahuja, S.S., McDaniel, B.L., Ahuja, S.K.

Notes: Reporter studies were performed in THP-1 monocytic cells, Jurkat T-cell leukemic cells and K562 myelogenous leukemia cells. Experimental promoter constructs were prepared in the pGL3 Basic Vector. Transfections were control by cotransfection with the pRL-CMV Vector, a source of Renilla luciferase. The activity of the two luciferases was determined with the Dual-Luciferase® Reporter Assay System. Ratios of pGL3-based Vectors to the pRL-CMV Vector were not reported but 0.5µg of the pRL-CMV Vector was used in each transfection. (0630)

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J. Biol. Chem. 272, 6979-6985. The rat arylalkylamine N-acetyltransferase gene promoter. cAMP activation via a cAMP-responsive element-CCAAT complex. 1997

Baler, R., Covington, S. and Klein, D.C.

Notes: Promega's Luciferase Assay System and pGL3 Basic Vector were used to study gene expression in primary rat pinealocytes. (1455)

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J. Biol. Chem. 272, 21090-21095. The rat glucocorticoid receptor mutant K461A differentiates between two different mechanisms of transrepression. 1997

Meyer, T., Starr, D.B., Carlstedt-Duke, J.

Notes: Luciferase studies were performed in HOS D4 osteosarcoma cells. Reporter constructs were prepared in the pGL2 Enhancer Vector. (0686)

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Proc. Natl. Acad. Sci. USA 94, 7400-7405. Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development. 1997

Tarutani, M., Itami, S., Okabe, M., Ikawa, M., Tezuka, T., Yoshikawa, K., Kinoshita, T., Takeda, J.

Notes: Luciferase studies were performed in PAM 212 mouse keratinocyte cells and NIH3T3 cells. They cloned a 14kb promoter fragment into the pGL3-Basic Vector and used this and the pGL3-Control Vector for their studies. (0303)

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J. Biol. Chem. 272, 23489-23497. Transcriptional regulation of the mouse presenilin-1 gene. 1997

Mitsuda, N., Roses, A.D. and Vitek, M.P.

Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)

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J. Biol. Chem. 272, 26360-26366. Transcriptional repression mediated by the PR domain zinc finger gene RIZ 1997

Xie, M. , Shao, G. , Buyse, I. M. , Huang, S.

Notes: Expression of luciferase reporter constructs, derived from the pGL3 Promoter Vector, was studied in 3T3 cells. (0165)

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J. Biol. Chem. 272, 30208-30214. Two naturally occurring insulin receptor tyrosine kinase domain mutants provide evidence that phosphoinositide 3-kinase activation alone is not sufficient for the mediation of insulin's metabolic and mitogenic effects. 1997

Krook, A., Whitehead, J.P., Dobson, S.P., Griffiths, M.R., Ouwens, M., Baker, C., Hayward, A.C., Sen, S.K., Maassen, J.A., Siddle, K., Tavaré, J.M., O'Rahilly, S.

Notes: CHO-K1 cells were transfected at ~80% confluency with four plasmids: (1) a CMV-driven vector expressing the wildtype or mutant human insulin receptor; (2) a pGL3-Basic-derived Vector containing five GAL4 binding sites upstream of the firefly luciferase reporter; (3) the pRL-CMV Control Vector; and (4) a fusion of the GAL4-binding domain and Elk-1 transcription factor. The Dual-Luciferase® Reporter Assay System was used to confirm that specific mutations in the insulin receptor negate its ability to activate the Elk-1 transcription factor. (0896)

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Hum. Gene Ther. 7, 1205-1217. An improved plasmid DNA expression vector for direct injection into skeletal muscle. 1996

Hartikka, J., Sawdey, M., Cornefert-Jensen, F., Margalith, M., Barnhart, K., Nolasco, M., Vahlsing, H.L., Meek, J., Marquet, M., Hobart, P., Norman, J., Manthorpe, M.

Notes: pGL3-Control Vector and the Luciferase Assay System were used for in vivo studies on murine quadricep muscles by injection of DNA. Extracts for assaying the tissues were prepared using the CCLR. (1066)

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Gene Ther. 3, 789-796. Effect of amniotic fluid on cationic lipid mediated transfection and retroviral infection. 1996

Douar, A. M. , Themis, M. , Sandig, V. , Friedmann, T. , Coutelle, C.

Notes: The Tfx™-50 Reagent was used to transfect pGL3 Vectors into NIH3T3 cells as well as microinjection into amniotic fluid (clone A31). (1243)

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