Citations Search

Notes: Luciferase studies were performed in AtT20 cells using the Luciferase Assay System. Experimental constructs were prepared in the pGL3 Basic Vector. (1392)

Notes: Luciferase and CAT assays were conducted in CHO-K1 cells using constructs prepared in the pGL3-Basic and pCAT^®-Basic Vectors, and the activity was normalized to β-galactosidase activity supplied by the pSV-β-Galactosidase Control Vector. The pCAT^® Vectors have been replaced by the next-generation pCAT^®3 Vectors. (1055)

Notes: Luciferase studies were performed in CA77 thyroid C cell line UI-0 using constructs prepared in the pGL3-Basic Vector. (0838)

Notes: pGL3 Basic Vector and pSV-β-galactosidase Control Vector were used in bovine aortic endothelial cells and rat vascular smooth muscle cells. The NF-κB p50 protein was used for DNase Footprinting (2-20 gel shift units). HeLaScribe^® Nuclear Extract, Gel Shift Grade, was used as a positive control for NF-κB in a gel shift assay. (0793)

Notes: Luciferase studies were performed in aortic and vena caval smooth muscle cells. Transfections were normalized to β-galactosidase activity. Researchers report that pGL3 Promoter and pGL3 Control Vectors were 55-± 3-fold and 340±41-fold greater activity, respectively, than the pGL3 Basic Vector alone. The CREB consensus oligo was used in gel shifts of aortic and vena cava smooth muscle cell nuclear extracts. (1214)

Notes: Luciferase studies were performed in KRC-7 rat hepatoma cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-galactosidase Vector. Luciferase activities determined with the Luciferase Assay System were normalized to readings from the Beta-Galactosidase Enzyme Assay System. (1364)

Notes: Expression of luciferase reporter constructs, derived from the pGL3 Vectors, was studied in human keratinocytes. (0166)

Notes: Expression of luciferase reporter constructs, derived from pGL3 Basic Vectors, was studied in MCF7, T47D, BT20, SKOV3 and JAR human cancer cell lines. The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer was also used. (0121)

Notes: Reporter studies were performed in HepG2 cells. The experimental constructs were made using the pGL3 Basic Vector and luciferase activities were monitored with the Luciferase Assay System. (0669)

Notes: Luciferase studies were performed in HeLa cells using constructs prepared in the pGL3 Basic Vector. (1339)

Notes: Studies performed in primary astrocytes. (1613)

Notes: Authors make 3 base changes in a promoter element using the Altered Sites^® II in vitro Mutagenesis System and put the mutated promoter into the pGL3-Basic Vector. Luciferase activity was measured using the Luciferase Assay System. (1047)

Notes: Luciferase studies were performed in 293T cells using constructs prepared in the pGL3 Basic Vector. (1278)

Notes: Chang liver cells were transfected with constructs in pGL3-Basic and pGL3-Promoter Vectors using the Tfx™-50 Reagent. Constructs were verified with the fmol^® DNA Cycle Sequencing System. (0911)

Notes: Promoter activity was assayed in JEG-3 choriocarcinoma cells, JAR choriocarcinoma cells, HeLa cells and primary adipocytes. The pGL3 Vectors were transfected at a 20:1 ratio over pRL-CMV Vector. Enhancer activity was functional only in the choriocarcinoma cells. The Dual-Luciferase^® Reporter Assay System was used to measure luciferase expression. (1446)

Notes: Expression of luciferase reporter constructs, derived from the pGL3-Basic and pGL3-Promoter Vectors, was studied in placental JEG-3 cells, U-2 OS osteogenic sarcoma cells, JAR human choriocarcinoma cells, TC1 thyroid carcinoma cells, HeLa cells, Hep3B cells, MCF-7 cells and AtT20 cells. (0207)

Notes: The authors used the Promega pSV-β-Galactosidase Control Vector, pGL3-Control and pGL3-Basic Vectors, Luciferase Assay Reagent, TNT^® Coupled Wheat Germ Extract Systems and TNT^® Coupled Reticulocyte Lysate Systems in their studies NF-κB. (0994)

Notes: The authors used Promega's  pGL3 Basic Vector to study promoter activity in primary keratinocytes. (0517)

Notes: Luciferase studies performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-Galactosidase Control Vector to normalize for transfection efficiency. Luciferase activities were measured with the Luciferase Assay System. (0629)

Notes: Expression of luciferase reporter constructs, derived from the pGL3-Basic Vector, was studied in COS-7 cells. (0177)

Notes: Luciferase studies were performed on extracts of transfected Hep3B, HepG2 and HeLa cells. The experimental constructs were prepared in the pGL3 Basic and pGL3 Promoter Vectors. (0452)

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM^®-T Vector and sequenced. (1491)

Notes: Luciferase studies were performed in HL-60 cells. Experimental constructs were prepared in pGL3 Basic Vector and luciferase activities determined with the Luciferase Assay System. (0796)

Notes: Authors performed luciferase assays using the pGL3 Basic Vector in Calu-6 cells. (1151)

Notes: Luciferase studies were performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. (1137)