BRET with Low Background and Increased Signal
The study of PPIs with BRET relies on energy transfer from luciferase (BRET donor) attached to one interacting partner, to a fluorescent BRET acceptor tagged to another interacting partner. The transfer of energy is moderated by the proximity of the two partners. The NanoBRET™ assay is a substantially improved BRET assay that uses NanoLuc® luciferase as the energy donor and HaloTag® Protein Labeled with the NanoBRET™ 618 fluorophore as the energy acceptor. The bright, blue-shifted signal from the NanoLuc® donor combined with the far-red-shifted HaloTag® acceptor create an assay with optimal spectral overlap, increased signal, and lower background compared to conventional BRET assays.
Design your own assay or choose from a large selection of prebuilt NanoBRET™ PPI assays targeting: bromodomain and other epigenetic proteins, transcriptional proteins, signaling protein and kinase assays, and membrane protein assays.