Protein interactions are critical to the majority of cellular processes, including: replication, transcription, translation and signal transduction. HaloTag technology provides a convenient method for characterization of protein interactions. The HaloTag® Mammalian Pull-Down Systems are designed to capture and purify intracellular binary and higher order protein complexes, including transient or weakly interacting partners.
- Identify more physiologically relevant protein partners
- Rapidly capture binary and higher order complexes directly from lysates
- Transition from pull-downs into imaging studies using the same construct
In the HaloTag® Mammalian Pull-Down System, the covalent binding of HaloTag®-fusion proteins affords efficient protein recovery, and low non-specific binding.
The HaloTag® Mammalian Pull-Down System gives consistent results and clean controls when pulling down macro-molecular complexes.
With the HaloTag® technology, you can seamlessly transition from pull-down studies to imaging applications without additional cloning as all HaloTag® Ligands are interchangeable. In the example below, U2OS cells stably expressing p65-HaloTag labeled with HaloTag TMRDirect™ Ligand show proper cytoplasmic localization.
"Examining the Complexity of Human RNA Polymerase Complexes..."
J Proteome Res., 2012 Jan. 3 (2013).