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How to Interrogate Epigenetic Events in Live Cells

Danette Daniels received her Ph.D. in biophysics from Yale University, then completed a postdoctoral fellowship at Stanford University School of Medicine, characterizing transcriptional regulation of the Wnt signaling pathway using X-ray crystallography and biochemical approaches.  She has been at Promega for over 10 years and is currently a Group Leader developing applications for studies of global protein:protein and protein:DNA interactions using NanoLuc® and HaloTag® technologies with an emphasis in epigenetics research.
  • Danette L Daniels, PhD

  • Sr Research Scientist

  • Original Webinar Date: Tuesday, January 10, 2017

Modification of gene expression rather than alteration of the genetic code (Epigenetics) is an interesting way that organisms develop a phenotype. Altered expression of the key proteins can have deleterious effects. Researchers are actively engaged in identifying modulators of epigenetic proteins and learning how these proteins interact with their targets. Learn more as we present data on the use of a novel BRET assay for such studies.

Epigenetics is the study of changes in phenotype caused by modification of gene expression rather than alteration of the genetic code itself. Bromodomain proteins, methyltransferases and demethylases, histone acetylases and deacetylases, are just a few proteins mediating the modification of gene expression. Aberrant expression of these and other important cellular proteins can lead to diseases such as cancer and inflammatory disorders. A significant goal of current research is to find modulators of epigenetic proteins and develop new therapeutics.

Researchers have made key discoveries in therapeutic epigenetic inhibitors recently. To better understand the mechanisms of action, we need to better elucidate how the inhibitors interact with their target proteins and identify any interacting partners, both on- and off- target. Here we present new approaches using BRET (bioluminescence resonance energy transfer) to directly measure drug-target interactions and proteins binding to chromatin within live cells.