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Recombinant Clone Screening Using the GoTaq Hot Start Green Master Mix

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Abstract

We describe using the new GoTaq® Hot Start Green Master Mix to screen recombinant clones from single colonies on a plate and from plasmid minipreps. The master mix formulation contains direct-to-gel loading components, which provide convenience and allow fast PCR setup. With the GoTaq® Hot Start Polymerase technology, reactions can be assembled and stored at room temperature for up to 24 hours prior to cycling.

Promega Notes 101, 13–14.

Sarah Wheeler

Promega Corporation

Publication Date: 2009

Download Article (577 KB)