GoTaq® Hot Start Green Master Mix Protocol
Literature # 9PIM512
GoTaq® Hot Start Green Master Mix is a premixed ready-to-use solution containing GoTaq® Hot Start Polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR. GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets, because it may eliminate or minimize primer-dimer and secondary products. In some cases, hot-start PCR may improve yields.