Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo® Plus Assay

Tracy Worzella and Brad Larson
Promega Corporation
Publication Date: 2006

Abstract

Here we demonstrate the concept of kinase profiling using a panel of four kinases and ten kinase inhibitors. We used the Kinase-Glo® Plus Luminescent Kinase Assay and the Deerac Fluidics™ Equator™ HTS non-contact liquid handling system to design and perform an ultrahigh-throughput profiling screen. We present IC50 values for each test compound and show how such profiling can streamline the initial drug discovery process.

Promega Notes 93, 2–4.