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Optimizing Kinase Assays for Ultrahigh-Throughput Profiling Using the Kinase-Glo® Plus Assay

Tracy Worzella and Brad Larson
Promega Corporation
Publication Date: 2006


Here we demonstrate the concept of kinase profiling using a panel of four kinases and ten kinase inhibitors. We used the Kinase-Glo® Plus Luminescent Kinase Assay and the Deerac Fluidics™ Equator™ HTS non-contact liquid handling system to design and perform an ultrahigh-throughput profiling screen. We present IC50 values for each test compound and show how such profiling can streamline the initial drug discovery process.

Promega Notes 93, 2–4.