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Measuring Renilla Luciferase Luminescence in Living Cells

Erika Hawkins1, James Unch2, Nancy Murphy1, Jolanta Vidugiriene1, Mike Scurria2, Dieter H. Klaubert2 and Keith V. Wood1
1Promega Corporation and 2Promega Biosciences, Inc.
Publication Date: 2005

Abstract

The ViviRen™ and EnduRen™ Live Cell Substrates have been developed to make measuring Renilla luciferase from live cells simpler and more sensitive than was possible with the substrates coelenterazine and coelenterazine-h. ViviRen™ Substrate generates very bright luminescence, at least 3-fold brighter than coelenterazine, while EnduRen™ Substrate generates very stable luminescence, permitting multiple measurements over incubations of 24 hours or more. Both live cell substrates can be multiplexed with CellTiter-Glo® Reagent to normalize for viable cell numbers. Alternatively, the Chroma-Luc™ red Luciferase can be used to normalize for viable cell number or transfection efficiency.

Promega Notes 90, 10–14.