Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Purification of In Vitro Transcribed RNA Using the SV Total RNA Isolation System

Marjeta Urh, Don Creswell, Jacqui Sankbeil, Dan Simpson, Rod Flemming, Cris Cowan and Gary Kobs
Promega Corporation
Publication Date: 2004

Abstract

Purification of RNA following in vitro transcription is required to remove unincorporated nucleotides that can affect concentration determination, to remove the enzymes used in the reaction and to exchange buffers. Traditional methods of RNA purification from in vitro transcription reactions have involved the use of phenol/chloroform extraction or lithium/chloride precipitation. These methods are often inefficient, and the yield is highly variable. Here we investigate using the SV Total RNA Isolation System for purification of RNA following in vitro transcription.

Promega Notes 86, 15–17.