GoTaq™ DNA Polymerase, Promega's new formulation of Taq DNA Polymerase, is supplied with two buffers—Green GoTaq™ Reaction Buffer and Colorless GoTaq™ Reaction Buffer. DNA amplified using GoTaq™ DNA Polymerase with Green GoTaq™ Reaction Buffer can be loaded directly onto agarose gels, eliminating the need to add loading dye or buffer to the PCR sample before electrophoresis. When PCR samples are not loaded onto a gel or when the dyes in the Green GoTaq™ Reaction Buffer would interfere with downstream applications, the Colorless GoTaq™ Reaction Buffer can be used.
Promega Notes 83, 21–24.
Amanda Glebs, Evelyn Stencel and Kimberly Knoche