Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Success

Thank you for verifying your email address.

Email Verification Error

There was an issue verifying your email address. Please try again or contact Customer Service.

Contact Customer Service

United States

Why does denatured DNA absorb more ultraviolet light than double-stranded DNA?

The phenomenon of UV absorbance increasing as DNA is denatured is known as the hyperchromic shift. The purine and pyrimidine bases in DNA strongly absorb ultraviolet light. Double-stranded DNA absorbs less strongly than denatured DNA due to the stacking interactions between the bases. Single deoxynucleotides absorb more strongly than denatured DNA(1).

Related Products

Related Resources

Article References

  1. Alexander, R.R. and Griffiths, J.M. (1993) Basic Biochemical Methods, 2nd Edition. J.M. Wiley and Sons, New York.

Products may be covered by pending or issued patents or may have certain limitations on use. Please visit our patent and trademark web page for more information.
Article Related Products Related Resources Related Articles Article References
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
Estonia
Finland
France
/
Germany
/
Iceland
Italy
/
Luxembourg
Netherlands
Norway
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Manage Cookies

Necessary Cookies

We use these cookies to ensure our site functions securely and properly; they are necessary for our services to function and cannot be switched off in our systems. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies.

Allow Preference Cookies

We use these cookies to remember your settings and preferences. For example, we may use these cookies to remember your language preferences.

Allow Performance/Statistics Cookies

We use these cookies to collect information about how you interact with our services and to help us measure and improve them. For example, we may use these cookies to determine if you have interacted with a certain page.

Allow Marketing Cookies

We and our advertising partners use these cookies to deliver advertisements, to make them more relevant and meaningful to you, and to track the efficiency of our advertising campaigns, both on our services and on other websites and social media.