The HaloTag® Interchangeable Labeling Technology provides new
options for rapid, site-specific labeling of proteins in living cells and in
vitro. The technology is based on the formation of a covalent bond between the
HaloTag® Protein and synthetic ligands that carry a variety of functionalities,
including fluorescent labels, affinity tags and attachments to a solid phase.
The covalent bond forms rapidly under physiological conditions, is highly
specific and essentially irreversible, yielding a complex that is stable even
under denaturing conditions. The ability to create labeled HaloTag® fusion
proteins with a wide range of optical properties and functions allows researchers to image and localize labeled HaloTag®; protein fusions in live- or fixed-cell populations and isolate and analyze HaloTag® Protein fusions and protein complexes. This article presents data obtained using three commercially available ligands and one ligand that is currently under
Cell Notes 11, 2–6.