The HaloTag® Interchangeable Labeling Technology provides new
options for rapid, site-specific labeling of proteins in living cells and in
vitro. The technology is based on the formation of a covalent bond between the
HaloTag® Protein and synthetic ligands that carry a variety of functionalities,
including fluorescent labels, affinity tags and attachments to a solid phase.
The covalent bond forms rapidly under physiological conditions, is highly
specific and essentially irreversible, yielding a complex that is stable even
under denaturing conditions. The ability to create labeled HaloTag® fusion
proteins with a wide range of optical properties and functions allows researchers to image and localize labeled HaloTag®; protein fusions in live- or fixed-cell populations and isolate and analyze HaloTag® Protein fusions and protein complexes. This article presents data obtained using three commercially available ligands and one ligand that is currently under
Cell Notes 11, 2–6.
Georgyi V. Los1, Al Darzins1, Natasha Karassina1, Chad Zimprich1, Randall Learish1, Mark G. McDougall2, Lance P. Encell1, Rachel Friedman-Ohana1, Monika Wood1, Gediminas Vidugiris1, Kris Zimmerman1, Paul Otto1, Dieter H. Klaubert2, and Keith V. Wood1