Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

HaloTag® Interchangeable Labeling Technology for Cell Imaging and Protein Capture

Georgyi V. Los1, Al Darzins1, Natasha Karassina1, Chad Zimprich1, Randall Learish1, Mark G. McDougall2, Lance P. Encell1, Rachel Friedman-Ohana1, Monika Wood1, Gediminas Vidugiris1, Kris Zimmerman1, Paul Otto1, Dieter H. Klaubert2, and Keith V. Wood1
1Promega Corporation, 2Promega Biosciences, Inc.
Publication Date: 2005


The HaloTag® Interchangeable Labeling Technology provides new options for rapid, site-specific labeling of proteins in living cells and in vitro. The technology is based on the formation of a covalent bond between the HaloTag® Protein and synthetic ligands that carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under physiological conditions, is highly specific and essentially irreversible, yielding a complex that is stable even under denaturing conditions. The ability to create labeled HaloTag® fusion proteins with a wide range of optical properties and functions allows researchers to image and localize labeled HaloTag®; protein fusions in live- or fixed-cell populations and isolate and analyze HaloTag® Protein fusions and protein complexes. This article presents data obtained using three commercially available ligands and one ligand that is currently under development.

Cell Notes 11, 2–6.

Related Products