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HaloTag® Interchangeable Labeling Technology for Cell Imaging and Protein Capture

Georgyi V. Los1, Al Darzins1, Natasha Karassina1, Chad Zimprich1, Randall Learish1, Mark G. McDougall2, Lance P. Encell1, Rachel Friedman-Ohana1, Monika Wood1, Gediminas Vidugiris1, Kris Zimmerman1, Paul Otto1, Dieter H. Klaubert2, and Keith V. Wood1
1Promega Corporation, 2Promega Biosciences, Inc.
Publication Date: 2005

Abstract

The HaloTag® Interchangeable Labeling Technology provides new options for rapid, site-specific labeling of proteins in living cells and in vitro. The technology is based on the formation of a covalent bond between the HaloTag® Protein and synthetic ligands that carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under physiological conditions, is highly specific and essentially irreversible, yielding a complex that is stable even under denaturing conditions. The ability to create labeled HaloTag® fusion proteins with a wide range of optical properties and functions allows researchers to image and localize labeled HaloTag®; protein fusions in live- or fixed-cell populations and isolate and analyze HaloTag® Protein fusions and protein complexes. This article presents data obtained using three commercially available ligands and one ligand that is currently under development.

Cell Notes 11, 2–6.

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