Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Comparing the Rapid Detection Protocol for the Nano-Glo® HiBiT Detection System with Traditional Epitope Tag Western Blotting

Publication Date: 6/20

Abstract

This Application Note demonstrates the sensitivity and time-saving advantages of the HiBiT tag for detection of membrane-immobilized proteins of interest in blots compared to traditional Western blotting methods that use an antibody to an epitope tag (e.g., FLAG® tag).

The experiments included here show that the HiBiT blotting system can provide greater sensitivity than epitope tag blotting, allowing shorter image acquisition times. Results also demonstrate higher signal-to-noise ratio and a significantly faster protocol with fewer hands-on steps for the HiBiT method.