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Real-Time PCR: Guidelines for a Comparison of Reagent Performance

Sarah Teter, PhD, and Leta Steffen, PhD
Promega Corporation
Publication Date: 5/17


A protocol for testing previously optimized qPCR assays with a new qPCR master mix to look at several factors of reagent performance including assay specificity, reproducibility, linearity, sensitivity and efficiency.

Application Note #AN299