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Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader

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Abstract

Promega has developed reporter bioassays that use activation of gene transcription through NFAT as an early measure of ADCC. These reporter bioassays provide a convenient system for assessing ADCC that uses frozen, thaw-and-use effector and target cells and eliminates the need for cell culture maintenance. Here we describe the assay as performed on the Infinite® 200 PRO Reader with gas control module and find it to be functionally equivalent to using a tissue culture incubator for the reporter induction phase of the ADCC Reporter Bioassay.

Kevin Schiro1, Tracy Worzella1, Zhijie Jey Cheng1, Denise Garvin1, Teresa Surowy1, Thomas Hengstl2, Michael Fejtl2, and Christian Oberdanner2

1Promega Corporation, 2Tecan Austria GmbH

Publication Date: April 2014

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How to Cite This Article

Schiro, K., Worzella, T., Cheng, Z., Garvin, D., Surowy, T., Hengstl, T., Fejtl, M. and Oberdanner, C. AN267-Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader. [Internet] April 2014. [cited: year, month, date]. Available from: http://www.promega.com/resources/pubhub/applications-notes/an267-performing-adcc-reporter-bioassay-with-an-environmentally-controlled-microplate-reader/

Schiro, K., Worzella, T., Cheng, Z., Garvin, D., Surowy, T., Hengstl, T., Fejtl, M. and Oberdanner, C. AN267-Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader. Promega Corporation Web site. http://www.promega.com/resources/pubhub/applications-notes/an267-performing-adcc-reporter-bioassay-with-an-environmentally-controlled-microplate-reader/ Updated April 2014. Accessed Month Day, Year.