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Purification of Concentrated High-Quality RNA from Cells and Tissues

Don Smith
Promega Corporation
Publication Date: 4/13

Abstract

The ReliaPrep™ RNA Miniprep Systems offer a complete solution for the isolation of quality RNA from very small amounts of cultured cells or tissues. They provide a rapid purification method with the option of elution in a small volume (15μl or less) to generate highly concentrated RNA. Purification is achieved without using phenol/chloroform extraction or beta-mercaptoethanol, and a simple DNase step is included to eliminate genomic DNA efficiently. In this article, we demonstrate that RNA isolated from cells and tissues using the ReliaPrep™ Systems is intact, highly pure and ideally suited for applications such as RT-qPCR.

How to Cite This Article

Scientific Style and Format, 7th edition, 2006

Smith, D. Purification of Concentrated High-Quality RNA from Cells and Tissues. [Internet] 4/13. [cited: year, month, date]. Available from: https://www.promega.com/resources/pubhub/applications-notes/an213-purification-of-concentrated-high-quality-rna-from-cells-and-tissues/

American Medical Association, Manual of Style, 10th edition, 2007

Smith, D. Purification of Concentrated High-Quality RNA from Cells and Tissues. Promega Corporation Web site. https://www.promega.com/resources/pubhub/applications-notes/an213-purification-of-concentrated-high-quality-rna-from-cells-and-tissues/ Updated 4/13. Accessed Month Day, Year.