The Spectrum CE System is an automated 8-capillary electrophoresis instrument with an expanded spectral capacity, supporting a range of commercially available STR systems including 5-, 6-, and next generation 8-dye PowerPlex® systems.
The Spectrum CE System uses electrokinetic injection to inject samples from eight wells of a 96-well plate into each of the eight capillaries in the instrument. As samples pass through the polymer-filled capillaries they are separated through the application of an electric current and subsequently detected using laser-excitation and light capture with an integrated camera system. The instrument then automatically replaces the polymer in each capillary and moves on to the next set of eight samples.
The system includes the instrument itself, a desktop computer and a monitor. It is equipped with Spectrum Control Software (SCS) that provides a user-friendly interface for simplified operation. Consumables are pre-filled and ready-to-use, removing the need for time-consuming manual preparation and reducing the chances of human error. The integrated RFID readers are used to capture RFID information on consumables for easy exchange and tracking. Users can monitor each run in real time and view results while data collection is in progress. Pre-installed on the system computer are the genotyping tools GeneMarker® HID for Spectrum CE Systems (GMHID-Spectrum) and HIDAuto for Spectrum CE Systems, which offer high-quality data analysis.
In-lab validation of fragment analysis on the Spectrum CE System demonstrated its performance following the standards and guidelines outlined in the FBI Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods Validation Guidelines for DNA Analysis Methods. Characteristics described in validation included baseline noise and threshold evaluation, precision, resolution, accuracy, reproducibility, sensitivity, signal variability, dye balance, spectral artifact analysis, contamination assessment, mixture samples and direct amplification.