Choosing the Right Transfection Reagent for Your Cell Type
Publication Date: 2014
Abstract
ViaFect™ Transfection Reagent. FuGENE® 6 Transfection Reagent. FuGENE® HD Transfection Reagent. All three reagents offer broad spectrum transfection capability and low toxicity, but the question often comes up: "Which one should I choose?" Not every transfection reagent is perfect for every cell line, and transfection success can also depend on other parameters such as DNA quality, passage number of cells, and cell density.
To get a better understanding of how each reagent performs on a variety of cell lines, we performed transfection experiments with ViaFect, FuGENE® 6, FuGENE® HD and Lipofectamine® 2000 (Thermo Fisher) transfection reagents. Each reagent was tested with a range of transfection reagent:DNA ratios and two concentrations of DNA.
Summary
All four transfection reagents work on a variety of cells. Each reagent clearly works best on certain cell lines but none were perfect for every cell line. The data presented in this article show that ViaFectâ„¢ Reagent yielded greater luciferase expression over a wider variety of cells than the other reagents tested.
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