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PowerPlex® 16 HS System for Use on the Applied Biosystems® Genetic Analyzers Technical Manual

Instructions for Use of Product(s)
DC2100, DC2101

Literature # TMD022

The PowerPlex® 16 HS System allows co-amplification and three-color detection of sixteen loci (fifteen STR loci and Amelogenin), including Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL); one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 loci is labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein(JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® 16 HS System provides all of the materials necessary for amplification of these loci, including hot-start Taq DNA polymerase, and is compatible with Applied Biosystems® 3500, 3500xL, 3130 and 3130xl Genetic Analyzers.

Summary of Changes

The following changes were made to the 4/22 revision of this document:
1. Removed mention of ABI PRISM® Genetic Analyzers.
2. Added ProFlex® PCR System and removed GeneAmp® PCR System 9600 thermal cycler.
3. Updated Section 6.J.
4. Removed original Sections 9.C and 9.F.
5. Revised Section 9.E.
6. Updated Table 4.
7. Removed disclaimers.
8. Incorporated other general updates.
9. Updated the cover page, including the document title.

Revised 4/22.