TransfectNow™ HEK293 Cells Technical Manual

Instructions for Use of Product(s)
NC1001, NC1002

Literature # TM690

Transient transfection is a convenient approach for executing cell-based assays in easily transfectable cell lines such as HEK293 cells. For this reason, NanoBRET™ cell-based assay platforms from Promega make extensive use of the transient transfection approach. However, the requirement to maintain cells in continuous culture to support routine transfection can be tedious. Here we introduce TransfectNow™ HEK293 Cells as a convenient and reliable alternative to using freshly cultured and harvested HEK293 cells.

Summary of Changes
The following changes were made to the 9/23 revision of this document:
1. Added the NanoBRET™ TE K192 Kinase Selectivity and NanoBRET™ TE CDK Selectivity Systems to Figure 1, Table 1 and Sections 2, 4 and 6.

Revised 9/23.