TLR Bioassay Technical Manual
Instructions for Use of Product(s)
JA9011, JA9015, JA9111, JA9115
Literature # TM705
Modulating the TLR system, either by agonism or antagonism, has the potential to modulate immune and inflammatory responses. Blocking TLRs may inhibit inflammatory responses, while TLR agonists may promote immune responses against pathogens or cancer. Current methods for assessing the activity of TLR modulators typically rely on primary monocyte-derived macrophages and measuring functional endpoints, such as cytokine production. These assays are laborious and highly variable due to their reliance on donor cells, complex assay protocols and unqualified assay reagents. As a result, these assays are difficult to establish in a quality-controlled setting.
The TLR Bioassay is a bioluminescent reporter cell-based assay that is easy-to-use, quantitative and demonstrates a functional readout of agents that activate TLRs. The TLR Bioassay can be used to measure the potency of agonists of TLRs 1, 2, 4, 5, 6, 7 and 8. It can also be used to measure the potency of inhibitors of TLRs. The assay consists of one genetically engineered cell line:
• TLR Bioassay Cells: Monocytic cells endogenously expressing TLRs 1, 2, 4, 5, 6, 7 and 8, with a stably integrated NanoLuc® luciferase reporter driven by TLR pathway-dependent response elements.
The TLR Bioassay Cells are provided in thaw-and-use format, as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation.
This Technical Manual is relevant for Cat.# JA9011 and JA9015; JA9111 and JA9115 (Korea).
Summary of Changes
The following changes were made to the 4/25 revision of this document:
1. Lumit trademark was updated to registered.
2. In Section 3, text about the product label was revised.
3. Expired patent statement was removed.
Revised 4/25.