Nano-Glo® Fluorofurimazine In Vivo Substrate Technical Manual
Instructions for Use of Product(s)
N4100, N4110
Literature # TM722
The Nano-Glo® Fluorofurimazine In Vivo Substrate is an optimized formulation for bioluminescence imaging of NanoLuc® luciferase, NanoLuc® fusion proteins or reconstituted NanoBiT® luciferase in animal models. The formulation contains fluorofurimazine (FFz), a furimazine derivative with increased aqueous solubility, and poloxamer-407 (P-407). This formulation provides increased substrate bioavailability in vivo, leading to signals that are substantially brighter than formulations containing furimazine. With NanoLuc® luciferase, the emission maximum is 459nm, similar to furimazine.
Summary of Changes
The following changes were made to the 3/24 revision of this document:
1. Added volume of recommended dose of fluorofurimazine reconstitution to Section 3.C.
2. Made minor text edits.
Revised 3/24.