Nano-Glo® Endurazine™ and Vivazine™ Live Cell Substrates Technical Manual
Literature # TM550
The Nano-Glo® Endurazine™ and Vivazine™ Live Cell Substrates are versions of furimazine that enable live-cell, nonlytic assays for periods lasting several hours or days. For both substrates, a slow rate of ester hydrolysis leads to the steady release of furimazine throughout the experiment, a process catalyzed by cellular esterases. Once formed, furimazine can serve as a substrate for NanoLuc® or NanoBiT® luciferases. NanoBiT® luciferase consists of Large BiT (LgBiT) bound to Small BiT (SmBiT) or HiBiT peptides. Both substrates contain a proprietary compound that reduces furimazine autoluminescence.
Printed in USA 8/18.