ReliaPrep™ FFPE gDNA Miniprep System Technical Manual
Literature # TM352
The ReliaPrep™ FFPE gDNA Miniprep System uses incubation conditions optimized to partially reverse cross-linking caused by formalin fixation without the need for an overnight digestion. This system also incorporates a deparaffinization method that does not rely on xylene or other hazardous or volatile solvents.
Summary of Changes
The following changes were made at the 12/15 revision of this document:
1. Blue Dye is now included in the ReliaPrep™ FFPE gDNA Miniprep System.
2. A step was added for preparing the Lysis Buffer (LBA) with the Blue Dye.
3. The lysis step in the method now includes a 4-hour incubation at 80°C instead of one hour.
4. An optional overnight storage step was added after the 80°C incubation of the lysis step.
Printed in USA. Revised 12/15.