Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Limited Functionality Warning

We are currently updating our network. During this time, certain functionality may be unavailable, including online orders. We apologize for any inconvenience this may cause you.

Please contact Customer Service with any questions or comments.
Phone: (608) 274-4330
Toll-Free Phone: (800) 356-9526
Email: custserv@promega.com
Hours: 7am – 6pm, CST, Monday-Friday

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

ReliaPrep™ FFPE gDNA Miniprep System Technical Manual

Instructions for Use of Product(s)
A2351, A2352

Literature # TM352

The ReliaPrep™ FFPE gDNA Miniprep System uses incubation conditions optimized to partially reverse cross-linking caused by formalin fixation without the need for an overnight digestion. This system also incorporates a deparaffinization method that does not rely on xylene or other hazardous or volatile solvents.

Summary of Changes
The following changes were made at the 12/15 revision of this document:
1. Blue Dye is now included in the ReliaPrep™ FFPE gDNA Miniprep System.
2. A step was added for preparing the Lysis Buffer (LBA) with the Blue Dye.
3. The lysis step in the method now includes a 4-hour incubation at 80°C instead of one hour.
4. An optional overnight storage step was added after the 80°C incubation of the lysis step.

Printed in USA. Revised 12/15.

Experienced User Protocols