PowerPlex® ESI 16 Fast System for Use on the Applied Biosystems® Genetic Analyzers Technical Manual
Literature # TMD044
The PowerPlex® ESI 16 Fast System allows co-amplification and four-color fluorescent detection of sixteen loci, including D22S1045, D2S1338, D19S433, D3S1358, Amelogenin, D2S441, D10S1248, D1S1656, D18S51, D16S539, D12S391, D21S11, vWA, TH01, FGA and D8S1179. The system provides all materials necessary to amplify STR regions of human genomic DNA, including a hot-start thermostable DNA polymerase. The PowerPlex® ESI 16 Fast System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Summary of Changes
The following changes were made to the 12/20 revision of this document:
1. Added instructions for use of the ProFlex® PCR System.
2. Updated Section 6.L.
3. Revised Section 7.
4. Incorporated other general updates.