ECL Western Blotting Substrate Technical Manual
Instructions for Use of Product(s)
W1001, W1015
Literature # TM317
The ECL Western Blotting Substrate is a highly sensitive non-radioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western Blotting Substrate detects picogram amounts of antigen, and with the use of photographic or other imaging methods, visualizes the presence of HRP. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and reprobed.
Summary of Changes
The following changes were made to the 10/25 revision of this document:
1. Updated Section 6.B.
2. Moved content into a new template with a new cover page and updated document fonts.
Revised 10/25