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CellTiter-Glo® 3D Cell Viability Assay Technical Manual

Instructions for Use of Product(s)
G9681, G9682, G9683

Literature # TM412

The CellTiter-Glo® 3D Cell Viability Assay is a homogeneous method to determine the number of viable cells in 3D cell culture based on quantitation of the ATP present, which is a marker for the presence of metabolically active cells. This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent.

The CellTiter-Glo® 3D Cell Viability Assay is formulated with more robust lytic capacity and is designed for use with microtissues produced in 3D cell culture. The assay is compatible with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS) using cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves addition of a single reagent (CellTiter-Glo® 3D Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required.


Summary of Changes
The following changes were made to the 1/23 revision of this document:
1. Legal statements and Related Products were updated.
2. “Assay Advantages” was removed.
3. References were updated to include a missing reference.
4. Cover image and font were updated.

Revised 1/23.