Wizard® Plus SV Minipreps DNA Purification System Protocol
Literature # TB225
The Wizard® Plus SV Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 30 minutes or less, depending on the number of samples processed. This system can be used to isolate any plasmid from E.coli hosts but works most efficiently when the plasmid is <20,000bp in size. Purified plasmids can be used without further manipulation and can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
The protocol presented in this Technical Bulletin is for the isolation of plasmid DNA from E. coli. Plasmid DNA can be purified from 1–10ml of overnight cultures of E. coli with the Wizard® Plus SV Minipreps DNA Purification System. The yield of plasmid will vary depending on a number of factors, including the volume of bacterial culture, plasmid copy number, type of culture medium and bacterial strain used.
Printed in USA. Revised 12/10.