Wizard® Magnetic 96 DNA Plant System Protocol
Literature # TB289
The Wizard® Magnetic 96 DNA Plant System eliminates lengthy incubations and the use of hazardous organic solvents such as chloroform and phenol while offering the ability to perform plant genomic DNA purification in a 96-well plate format. These procedures can be performed manually or automated on a Beckman Coulter Biomek® 2000 workstation. The Wizard® Magnetic 96 DNA Plant System purifies DNA from commercially important agricultural crops such as corn and tomato leaf as well as canola and sunflower seeds. DNA purified from these samples can be used in PCR as well as more stringent applications such as RAPD analysis. The Wizard® Magnetic 96 DNA Plant System uses MagneSil® Paramagnetic Particles (PMPs). Paramagnetic particles can be considered a "mobile solid phase". Unlike column-based systems, binding of nucleic acids to magnetic particles can occur in solution, resulting in increased binding kinetics and binding efficiency.
Summary of Changes
The following changes were made to the 5/17 revision of this document:
1. Discontinued products were removed from Related Products.
2. The document design was updated.
Printed in USA. Revised 5/17