SV 96 Total RNA Isolation System Protocol
Literature # TB294
The SV 96 Total RNA Isolation System provides a fast, simple technique to purify intact RNA from tissue culture cells. This technique also allows automation on liquid-handling workstations such as the Beckman Coulter Biomek® 2000. Total RNA can be purified from 96 samples at once in less than an hour. The system incorporates a DNase-treatment step that substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol/chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.
Summary of Changes
The following changes were made to the 8/16 revision of this document:
1. The amount and concentration of the β-Mercaptoethanol component was changed, Sections 2 and 4.A.
2. The document design was updated.
Printed in USA. Revised 8/16.