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GoTaq® PCR Core System Technical Bulletin

Instructions for Use of Product(s)

Literature # TB254

The GoTaq® PCR Core System provides all of the reagents necessary for exponential amplification of specific regions of DNA using the polymerase chain reaction (PCR). The GoTaq® PCR Core System is supplied with GoTaq® DNA Polymerase, a proprietary formulation of Taq DNA polymerase designed for enhanced amplification, and GoTaq® Flexi Buffers, magnesium-free proprietary buffer formulations supplied at pH 8.5.

Although PCR is simple in theory, it can benefit from optimization of several parameters. This technical bulletin provides important and convenient information about performing PCR and includes protocols and troubleshooting tips for successful amplification.

Summary of Changes
The following changes were made to the 5/19 revision of this document:
1. The document design was updated.
2. Product and related product information were updated.

Printed in USA. Revised 5/19

Experienced User Protocols