pNLF1-HIF1a [CMV/neo] Vector
Instructions for Use of Product(s)
Literature # 9PIN138
The pNLF1-HIF1a [CMV/neo] Vector encodes a HIF1A-NanoLuc® fusion protein under control of a mammalian (CMV) promoter. The NanoLuc® gene encodes an engineered luciferase optimized for extreme brightness, small size and robust thermostability. The vector backbone contains a neomycin phosphotransferase gene to allow selection in E. coli with kanamycin and in mammalian cell lines with neomycin. This product also includes Transfection Carrier DNA for dilution of the reporter DNA, allowing titratable intracellular expression of the HIF1A-NanoLuc® fusion protein.