LigaFast™ Rapid DNA Ligation System Protocol
Literature # 9PIM822
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of cohesive-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no ATP or Mg2+ addition prior to use.
Printed in USA. Revised 10/16.