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pGL4.71[hRlucP] Vector Protocol

Instructions for Use of Product(s)
E6891

Literature # 9PIE689

The pGL4.71[hRlucP] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.71[hRlucP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucP luciferase reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

Printed in USA. Revised 9/16