Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

pGL4.50[luc2/CMV/Hygro] Vector

Instructions for Use of Product(s)

Literature # 9PIE131

The pGL4.50[luc2/CMV/Hygro] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis), which has been codon optimized for mammalian expression. This vector also is engineered with fewer consensus regulatory sequences for reduced backgrounds and a decreased risk of anomalous transcription. This vector contains the following features: luc2 reporter gene for expression in mammalian cells; CMV promoter for high translational expression; SV40 late poly(A) signal sequence is positioned downstream of luc2 to provide efficient transcription termination and mRNA polyadenylation; Binding region for RV primer 3 and RV primer 4; Synthetic poly(A) signal/transcription start site; Synthetic Hygromycin B-resistance gene for mammalian cell selection of the plasmid; Plasmid replication origin; Ampr gene for bacterial selection for vector amplification.

Printed in USA. Revised 10/16.