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pGL4.31[luc2P/Gal4UAS/Hygro] Vector Protocol

Instructions for Use of Product(s)

Literature # 9PIC935

The pGL4.31[luc2P/Gal4UAS/Hygro] Vector is designed to report transcriptional activation using the firefly luciferase reporter gene. This reporter vector contains five consensus binding sequences, or Upstream Activating Sequences, for the Gal4 DNA-binding domain (Gal4UAS) and a minimal adenoviral promoter upstream of the firefly luciferase gene. The vector backbone is based on the pGL4 luciferase reporter vector series, which has been engineered to reduce the occurrence of anomalous transcription. The luc2P reporter gene is a synthetic firefly luciferase sequence, which has been codon-optimized for high mammalian expression and contains minimal cryptic DNA regulatory elements. In addition, the luc2P gene incorporates a protein degradation sequence, PEST, resulting in a destabilized reporter protein with a faster response to transcriptional regulation. The vector also contains an ampicillin selection marker (Amp) for E. coli and a hygromycin selection marker (Hygro) for mammalian cells. The pGL4.31[luc2P/Gal4UAS/Hygro] Vector is provided for use with the CheckMate™/Flexi® Vector Mammalian Two-Hybrid System (Cat.# C9360).

Printed in USA. Revised 10/16.