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pGL4.16[luc2CP/Hygro] Vector Protocol

Instructions for Use of Product(s)

Literature # 9PIE671

The pGL4.16[luc2CP/Hygro] Vector encodes the luciferase reporter gene luc2CP (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. This vector is also engineered with fewer consensus regulatory sequences for reduced background and a decreased risk of anomalous transcription and has a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.16[luc2CP/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2CP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by luc2CP responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.

Printed in USA. Revised 10/16.