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pGL4.14[luc2/Hygro] Vector Protocol

Instructions for Use of Product(s)

Literature # 9PIE669

The pGL4.14[luc2/Hygro] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene, which has been codon optimized for mammalian expression. The pGL4.14[luc2/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.

Printed in USA. Revised 10/16.